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Problems of Forensic Sciences

Screening method for the analysis of blood and urine for the presence of naturally occurring toxic compounds from mushrooms (fungi) using the LC-MS method

Data publikacji: 29.03.2022

Problems of Forensic Sciences (Z Zagadnień Nauk Sądowych), 2021, 126-127, s. 137 - 151

https://doi.org/10.4467/12307483PFS.20.008.15448

Autorzy

,
Bartłomiej Feigel
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
Wszystkie publikacje autora →
,
Dariusz Zuba
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
https://orcid.org/0000-0001-5133-1756 Orcid
Wszystkie publikacje autora →
Wojciech Lechowicz
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
https://orcid.org/0000-0002-5794-0373 Orcid
Wszystkie publikacje autora →

Abstrakt

Analysis of biological material collected during autopsies and even from living humans for the presence of amanitins and other fungal toxins remains a challenge in forensic toxicology. A qualitative method for the detection of α-amanitin, β-amanitin, γ-amanitin, muscarine, and psilocin in blood and urine has been developed. To achieve this goal, solid phase extraction HLB 3cc 60mg columns were used. Blood and urine samples were purified with water and aqueous methanol solution, and then extracted with acetonitrile. An LC/QTOF system equipped with a C18 column was applied to identify the analytes. Acetonitrile and water with formic acid were utilized as mobile phases. The developed method was validated. The detection limits for α-amanitin, β-amanitin, γ-amanitin, muscarine and psilocin are, respectively, 1.4 ng/ml, 0.3 ng/ml, 1.2 ng/ml, 1.8 ng/ml, and 0.3 ng/ml in blood, and 1.5 ng/ml, 2.1 ng/ml, 1.5 ng/ml, 1.6 ng/ml, and 1.1 ng/ml in urine. The developed method allows for efficient, qualitative identification of all the above-mentioned compounds in a toxicological laboratory.

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Informacje

Informacje: Problems of Forensic Sciences (Z Zagadnień Nauk Sądowych), 2021, s. 137 - 151

Typ artykułu: Oryginalny artykuł naukowy

Tytuły:

Angielski:

Screening method for the analysis of blood and urine for the presence of naturally occurring toxic compounds from mushrooms (fungi) using the LC-MS method

Autorzy

Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków

https://orcid.org/0000-0001-5133-1756

Dariusz Zuba
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
https://orcid.org/0000-0001-5133-1756 Orcid
Wszystkie publikacje autora →

Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków

https://orcid.org/0000-0002-5794-0373

Wojciech Lechowicz
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
https://orcid.org/0000-0002-5794-0373 Orcid
Wszystkie publikacje autora →

Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków

Publikacja: 29.03.2022

Otrzymano: 07.06.2021

Zaakceptowano: 02.11.2021

Status artykułu: Otwarte __T_UNLOCK

Licencja: CC BY-NC-ND  ikona licencji

Udział procentowy autorów:

Bartłomiej Feigel (Autor) - 33%
Dariusz Zuba (Autor) - 33%
Wojciech Lechowicz (Autor) - 34%

Korekty artykułu:

-

Języki publikacji:

Angielski, Polski

Liczba wyświetleń: 330

Liczba pobrań: 685