Screening method for the analysis of blood and urine for the presence of naturally occurring toxic compounds from mushrooms (fungi) using the LC-MS method
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RIS BIB ENDNOTEScreening method for the analysis of blood and urine for the presence of naturally occurring toxic compounds from mushrooms (fungi) using the LC-MS method
Data publikacji: 29.03.2022
Problems of Forensic Sciences (Z Zagadnień Nauk Sądowych), 2021, 126-127, s. 137 - 151
https://doi.org/10.4467/12307483PFS.20.008.15448Autorzy
Screening method for the analysis of blood and urine for the presence of naturally occurring toxic compounds from mushrooms (fungi) using the LC-MS method
Analysis of biological material collected during autopsies and even from living humans for the presence of amanitins and other fungal toxins remains a challenge in forensic toxicology. A qualitative method for the detection of α-amanitin, β-amanitin, γ-amanitin, muscarine, and psilocin in blood and urine has been developed. To achieve this goal, solid phase extraction HLB 3cc 60mg columns were used. Blood and urine samples were purified with water and aqueous methanol solution, and then extracted with acetonitrile. An LC/QTOF system equipped with a C18 column was applied to identify the analytes. Acetonitrile and water with formic acid were utilized as mobile phases. The developed method was validated. The detection limits for α-amanitin, β-amanitin, γ-amanitin, muscarine and psilocin are, respectively, 1.4 ng/ml, 0.3 ng/ml, 1.2 ng/ml, 1.8 ng/ml, and 0.3 ng/ml in blood, and 1.5 ng/ml, 2.1 ng/ml, 1.5 ng/ml, 1.6 ng/ml, and 1.1 ng/ml in urine. The developed method allows for efficient, qualitative identification of all the above-mentioned compounds in a toxicological laboratory.
Informacje: Problems of Forensic Sciences (Z Zagadnień Nauk Sądowych), 2021, 126-127, s. 137 - 151
Typ artykułu: Oryginalny artykuł naukowy
Tytuły:
Screening method for the analysis of blood and urine for the presence of naturally occurring toxic compounds from mushrooms (fungi) using the LC-MS method
Screening method for the analysis of blood and urine for the presence of naturally occurring toxic compounds from mushrooms (fungi) using the LC-MS method
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
Publikacja: 29.03.2022
Otrzymano: 07.06.2021
Zaakceptowano: 02.11.2021
Status artykułu: Otwarte
Licencja: CC BY-NC-ND
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Angielski, PolskiLiczba wyświetleń: 476
Liczba pobrań: 827