FAQ

Problems of Forensic Sciences

Evaluation of the Investigator 26plex QS and SureID PanGlobal systems in forensic analysis and comparison with commonly used NGM PCR Amplification Kit

Data publikacji: 08.09.2022

Problems of Forensic Sciences (Z Zagadnień Nauk Sądowych), 2022, 129, s. 93 - 103

https://doi.org/10.4467/12307483PFS.22.005.16306

Autorzy

,
Magdalena Marcińska
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
https://orcid.org/0000-0001-7616-7462 Orcid
Wszystkie publikacje autora →
,
Agnieszka Parys-Proszek
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
https://orcid.org/0000-0002-8321-6143 Orcid
Wszystkie publikacje autora →
Maria Wróbel
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
https://orcid.org/0000-0002-3168-5810 Orcid
Wszystkie publikacje autora →

Abstrakt

The aim of the study was to evaluate the novel kits – Investigator 26plex QS and SureID PanGlobal in forensic analysis and confront with commonly applied NGM PCR Amplification Kit. The internal validation study involved sensitivity, reliability, stochastic, DNA mixtures, inhibition parameters analysis and 15 casework samples extracted from blood, saliva, semen, tooth, bones, soft tissue and epithelial cells. The sensitivity experiments were performed on a total of 24 samples from seven serial dilutions of 007 control DNA. Mixture analysis was performed for two persons in different proportions using 9947 and 007 control DNA. Inhibition effect was tested using PCR inhibitor – humic acid. Among three compared kits the highest sensitivity was noted for Investigator 26plex QS considering the total number of the markers in each system, as well as the shared set of markers. However, low PCR efficiency of D6S1043 locus was observed with aforementioned kit. Mixture analysis performed with Investigator 26plex QS, SureID Pan Global and NGM kits, considering shared set of markers revealed that NGM Kit is the most sensitive for detecting minor components in a mixture. Similarly, we demonstrated that NGM is the most successful in the analysis of challenging forensic samples. The internal validation showed that the Investigator 26plex QS and SureID PanGlobal have weaker performance for routine forensic application in comparison to NGM Kit. However, thanks to increased number of markers, these systems could be helpful in analysis of complex cases in paternity testing and kinship analysis, in which standard genetic analysis is insufficient.

Acknowledgments
The authors would like to gratefully acknowledge the members of Qiagen and Health Gene Technologies groups for the financial support of this research. 

Bibliografia

1. Alsafiah, H. M., Aljanabi, A. A., Hadi, S., Alturayeif, S. S., Goodwin, W. (2019). An evaluation of the SureID 23comp Human Identification Kit for kinship testing. Nature Scientific Reports, 9(1), 16859.
2. Butler, J. M. (2015). Advanced topics in forensic DNA typing: Interpretation. Boston: Elsevier.
3. Butler, J. M. (2006). Genetics and genomics of core short tandem repeat loci used in human identity  testing. Journal of Forensic Sciences, 51, 253–265.
4. Carboni, I., Iozzi, S., Nutini, A. L., Torricelli, F., Ricci, U. (2014). Improving complex kinship analyses with additional STR loci. Electrophoresis, 35, 3145–3151.
5. Council Resolution of November 2009 on the exchange of DNA analysis results (2009). Official Journal of the European Communities C, 296(44), 1–4.
6. ENFSI DNA Working Group. (2010). Recommended Minimum Criteria for the Validation of Various Aspects of the DNA Profiling Process.
7. Investigator ®26plex QS Handbook. 04/2019.
8. Liu, Y., Guo, L., Jin, H., Li, Z., Bai, R., Shi, M., Ma, S. (2017). Developmental validation of a 6-dye typing system with 27 loci and application in Han population of China. Nature Scientific Reports, 7, 4706.
9. Parys-Proszek, A., Wróbel, M., Marcińska, M., Kupiec, T. (2018). Dual amplification strategy for improved efficiency of forensic DNA analysis using NGM Detect™, NGM™ or Globalfiler™ kits. Forensic Science International: Genetics, 35, 46–49.
10. Scientific Working Group on DNA Analysis Methods. (2016). SWGDAM validation guidelines for DNA analysis methods. SWGDAM.
11. User Guide SureID® Pan Global Human DNA Identification Kit. www.healthgenetech.com.
12. Vraneš, M., König, M., Cornelius, S., Kohns, A., Elliott, K., Prochnow, A., Scherer, M. (2019). Development of the Investigator®26plex QS kit: A new multiplex PCR kit for global STR analysis. Forensic Science International: Genetics Supplement Series, 7, 778–779.
13. Zgonjanin, D., Perruccio Soler, M., Antov, M., Redhead, P. (2017). Validation and implementation of the Investigator ®24plex QS Kit for forensic casework. Forensic Science International: Genetics Supplement Series, 6, 77–79.

Informacje

Informacje: Problems of Forensic Sciences (Z Zagadnień Nauk Sądowych), 2022, s. 93 - 103

Typ artykułu: Oryginalny artykuł naukowy

Tytuły:

Angielski:

Evaluation of the Investigator 26plex QS and SureID PanGlobal systems in forensic analysis and comparison with commonly used NGM PCR Amplification Kit

Autorzy

https://orcid.org/0000-0001-7616-7462

Magdalena Marcińska
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
https://orcid.org/0000-0001-7616-7462 Orcid
Wszystkie publikacje autora →

Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków

https://orcid.org/0000-0002-8321-6143

Agnieszka Parys-Proszek
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
https://orcid.org/0000-0002-8321-6143 Orcid
Wszystkie publikacje autora →

Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków

https://orcid.org/0000-0002-3168-5810

Maria Wróbel
Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków
https://orcid.org/0000-0002-3168-5810 Orcid
Wszystkie publikacje autora →

Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie, Kraków

Publikacja: 08.09.2022

Otrzymano: 14.03.2022

Zaakceptowano: 07.06.2022

Status artykułu: Otwarte __T_UNLOCK

Licencja: CC BY-NC-ND  ikona licencji

Udział procentowy autorów:

Magdalena Marcińska (Autor) - 33%
Agnieszka Parys-Proszek (Autor) - 33%
Maria Wróbel (Autor) - 34%

Korekty artykułu:

-

Języki publikacji:

Angielski, Polski

Liczba wyświetleń: 323

Liczba pobrań: 278