Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein
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RIS BIB ENDNOTESelenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein
Publication date: 05.08.2021
Acta Protozoologica, 2021, Volume 60, pp. 21 - 29
https://doi.org/10.4467/16890027AP.21.003.14063Authors
Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein
Selenoproteins have been described in all three domains of life and their function has been mainly associated with oxidative stress defense. Canonical elements required for selenoprotein production have been identified in members of the kinetoplastid group supporting the existence of a complete selenocysteine synthesis pathway in these organisms. Currently, nothing is known regarding the selenocysteine pathway in Trypanosoma evansi. In this study, we identified the expression of the elements selB, selC, selD, PSTK and selTRYP at the mRNA level in T. evansi. All translated proteins (selD, PSTK, selTRYP and selB) have the domains predicted and higher identity with Trypanosoma brucei. gambiense. The selenophosphate synthetase protein was localized in the cytoplasm. Our results support the existence of an active selenocysteine pathway in T. evansi.
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Information: Acta Protozoologica, 2021, Volume 60, pp. 21 - 29
Article type: Original article
Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil
Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil
Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis. SC. Brazil
Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis. SC. Brazil
Instituto Federal de Santa Catarina (IFSC), Campus Lages, Brazil
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
Published at: 05.08.2021
Article status: Open
Licence: CC BY
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