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Acta Protozoologica

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Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein

Publication date: 05.08.2021

Acta Protozoologica, 2021, Volume 60, pp. 21 - 29

https://doi.org/10.4467/16890027AP.21.003.14063

Authors

,
Kaio Cesar Simiano Tavares
Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
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,
Maria Gabriela Casagrande Dambrós
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
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,
André Saraiva Leão Antunes
Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil
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,
Pietro Martin Danziato
Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil
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,
Patricia Hermes Stoco
Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis. SC. Brazil
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,
Aline Daiane Schlindwein
Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis. SC. Brazil
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,
Renato Simões Moreira
Instituto Federal de Santa Catarina (IFSC), Campus Lages, Brazil
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
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Luiz Claudio Miletti
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
https://orcid.org/0000-0001-5926-0286 Orcid
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Abstract

Selenoproteins have been described in all three domains of life and their function has been mainly associated with oxidative stress defense. Canonical elements required for selenoprotein production have been identified in members of the kinetoplastid group supporting the existence of a complete selenocysteine synthesis pathway in these organisms. Currently, nothing is known regarding the selenocysteine pathway in Trypanosoma evansi. In this study, we identified the expression of the elements selBselCselDPSTK and selTRYP at the mRNA level in T. evansi. All translated proteins (selD, PSTK, selTRYP and selB) have the domains predicted and higher identity with Trypanosoma brucei. gambiense. The selenophosphate synthetase protein was localized in the cytoplasm. Our results support the existence of an active selenocysteine pathway in T. evansi.

References

Aeby E., Palioura S., Pusnik M., et al. (2009a) The canonical pathway for selenocysteine insertion is dispensable in Trypanosomes. Proc. Natl. Acad. Sci. USA 106: 5088–92. https://doi.org/10.1073/pnas.0901575106
https://doi.org/10.1073/pnas.0901575106
Aeby E., Seidel V., Schneider A. (2009b) The selenoproteome is dispensable in bloodstream forms of Trypanosoma brucei. Mol. Biochem. Parasitol. 168: 191–193. https://doi.org/10.1016/j.molbiopara.2009.08.007
https://doi.org/10.1016/j.molbiopara.2009.08.007
Aeby E., Ullu E., Yepiskoposyan H., et al. (2010) tRNASec is transcribed by RNA polymerase II in Trypanosoma brucei but not in humans. Nucleic Acids. Res. 38: 5833–5843. https://doi.org/10.1093/nar/gkq345
https://doi.org/10.1093/nar/gkq345
Allmang C., Wurth L., Krol A. (2009) The selenium to selenoprotein pathway in eukaryotes: more molecular partners than anticipated.
Biochim. Biophys. Acta 1790: 1415–23. https://doi.org/10.1016/j.bbagen.2009.03.003
https://doi.org/10.1016/j.bbagen.2009.03.003
Bonilla M., Krull E., Irigoín F., et al. (2016) Selenoproteins of African trypanosomes are dispensable for parasite survival in a mammalian host. Mol. Biochem. Parasitol. 206: 13–19. https://doi.org/10.1016/j.molbiopara.2016.03.002
https://doi.org/10.1016/j.molbiopara.2016.03.002
Bordo D., Bork P. (2002) The rhodanese/Cdc25 phosphatase superfamily. Sequence-structure-function relations. EMBO Rep. 3: 741–746
Bouzaidi-Tiali N., Aeby E., Charrière F., et al. (2007) Elongation factor 1a mediates the specificity of mitochondrial tRNA import in T. brucei. EMBO J. 26: 4302–12. https://doi.org/10.1038/sj.emboj.7601857
https://doi.org/10.1038/sj.emboj.7601857
Bulteau A. L., Chavatte L. (2015) Update on Selenoprotein Biosynthesis. Antioxid. Redox. Signal. 23: 775–794. https://doi.org/10.1089/ars.2015.6391
https://doi.org/10.1089/ars.2015.6391
Carlson B. A., Xu X. M., Kryukov G. V., et al. (2004) Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase. Proc. Natl. Acad. Sci. USA 101: 12848–12853. https://doi.org/10.1073/pnas.0402636101
https://doi.org/10.1073/pnas.0402636101
Carnes J., Anupama A., Balmer O., et al. (2015) Genome and Phylogenetic Analyses of Trypanosoma evansi Reveal Extensive Similarity to T. brucei and Multiple Independent Origins for Dyskinetoplasty. PLoS Negl. Trop. Dis. 9:e3404. https://doi.org/10.1371/journal.pntd.0003404
https://doi.org/10.1371/journal.pntd.0003404
Cassago A., Rodrigues E. M., Prieto E. L., et al. (2006) Identification of Leishmania selenoproteins and SECIS element. Mol. Biochem. Parasitol. 149: 128–134. https://doi.org/10.1016/j.molbiopara.2006.05.002
https://doi.org/10.1016/j.molbiopara.2006.05.002
Colpo C. B., Monteiro S. G., Stainki D. R., Colpo E. T. B, Henriques G. B. (2005) Infecção natural por Trypanosoma evansi em cães. Ciên. Rur. 35, 717–719
Costa F. C., Oliva M. A. V, De Jesus T. C. L., et al. (2011) Oxidative stress protection ofTrypanosomes requires selenophosphate synthase. Mol. Biochem. Parasitol. 180: 47–50. https://doi.org/10.1016/j.molbiopara.2011.04.007
https://doi.org/10.1016/j.molbiopara.2011.04.007
Desquesnes M., Dargantes A., Lai D-H, et al. (2013) Trypanosoma evansi and Surra: A Review and Perspectives on Transmission, Epidemiology and Control, Impact, and Zoonotic Aspects. Biomed. Res. Int. 2013: 1–20. https://doi.org/10.1155/2013/321237
https://doi.org/10.1155/2013/321237
Dikiy A., Novoselov S. V., Fomenko D. E., et al. (2007) SelT, SelW, SelH, and Rdx 12: Genomics and molecular insights into the functions of selenoproteins of a novel thioredoxin-like family. Biochemistry 46: 6871–6882. https://doi.org/10.1021/bi602462q
https://doi.org/10.1021/bi602462q
Duarte D. P., Tavares K. C. S., Lazarrotto C. R., et al. (2014) Genetic Profile of Two isolates of Trypanosoma evansi from Southern Brazil with different parasitaemias. Biotemas 27: 73–80. https://doi.org/10.5007/2175-7925.2014v27n3p73
https://doi.org/10.5007/2175-7925.2014v27n3p73
Geslain R., Aeby E., Guitart T., et al. (2006) Trypanosoma seryltRNA synthetase is a metazoan-like enzyme with high affinity for tRNASec. J. Biol. Chem. 281: 38217–38225. https://doi.org/10.1074/jbc.M607862200
https://doi.org/10.1074/jbc.M607862200
Grab D. J., Bwayo J. J. (1982) Isopycnic isolation of African trypanosomes on Percoll gradients formed in situ. Acta Trop. 39: 363–366
Itoh Y., Chiba S., Sekine S. I., Yokoyama S. (2009a) Crystal structure of human selenocysteine tRNA. Nucleic Acids Res. 37: 6259–6268. https://doi.org/10.1093/nar/gkp648
https://doi.org/10.1093/nar/gkp648
Itoh Y., Sekine S. ichi, Matsumoto E., et al. (2009b) Structure of Selenophosphate Synthetase Essential for Selenium Incorporation into Proteins and RNAs. J. Mol. Biol. 385: 1456–1469. https://doi.org/10.1016/j.jmb.2008.08.042
https://doi.org/10.1016/j.jmb.2008.08.042
Jensen R. E., Simpson L., Englund P. T. (2008) What happens when Trypanosoma brucei leaves Africa. Trends Parasitol. 24: 428–431
Joshi P. P., Shegokar V. R., Powar R. M., et al. (2005) Human trypanosomiasis caused by Trypanosoma evansi in India: the first case report. Am. J. Trop. Med. Hyg. 73: 491–5
Kumar R., Jain S., Kumar S., Sethi K., Kumar S., Tripathi B. N. (2017) Impact estimation of animal trypanosomosis (surra) on livestock productivity in India using simulation model: current and future perspective. Vet Parasitol Reg Stud Reports 10: 1–12
Koonin E. V., Aravind L., Leipe D. D., Iyer L. M. (2004). Evolutionary history and higher order classification of AAA ATPases. J. Struct. Biol. 146 (1–2): 11–31. doi:10.1016/j.jsb.2003.10.010
https://doi.org/10.1016/j.jsb.2003.10.010
Lanham S. M., Godfrey D. G. (1970) Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Exp. Parasitol. 28: 521–534. https://doi.org/10.1016/0014-4894(70)90120-7
https://doi.org/10.1016/0014-4894(70)90120-7
Leinfelder W., Forchhammer K., Zinoni F., Sawers G., Mandrand-Berthelot M. A., Böck A. (1988) Escherichia coli genes whose products are involved in selenium metabolism. J. Bacteriol. 170(2): 540–6
Lobanov A. V., Gromer S, Salinas G., Gladyshev V. N. (2006)
Selenium metabolism inTrypanosoma: Characterization of selenoproteomes and identification of a Kinetoplastida-specific selenoprotein. Nucleic Acids Res. 34: 4012–4024. https://doi.org/10.1093/nar/gkl541
https://doi.org/10.1093/nar/gkl541
Lun Z. R., Fang Y., Wang C. J., Brun R. (1993) Trypanosomiasis of domestic animals in China. Parasitol. Today 9: 41–45
Mangiapane E., Pessione A., Pessione E. (2014) Selenium and selenoproteins: an overview on different biological systems. Curr. Protein. Pept. Sci. 15: 598–607
Mariotti M., Salinas G., Gabaldón T., Gladyshev V. N. (2019) Utilization of selenocysteine in early-branching fungal phyla. Nat. Microbiol. 4(5): 759–765
Mueller E. J., Oh S., Kavalerchik E., et al. (1999) Investigation of the ATP binding site of Escherichia coli aminoimidazole ribonucleotide synthetase using affinity labeling and site-directed mutagenesis. Biochemistry 38: 9831–9839. https://doi.org/10.1021/bi990638r
https://doi.org/10.1021/bi990638r
Papp L. V, Lu J., Holmgren A., Khanna K. K. (2007) From selenium to selenoproteins: synthesis, identity, and their role in human health. Antioxid. Redox. Signal. 9: 775–806. https://doi.org/10.1089/ars.2007.1528
https://doi.org/10.1089/ars.2007.1528
Richardson J. B., Lee K. Y., Mireji P., et al. (2017) Genomic analyses of African Trypanozoonstrains to assess evolutionary relationships and identify markers for strain identification. PLoS Negl. Trop. Dis. 11:e0005949. https://doi.org/10.1371/journal.pntd.0005949
https://doi.org/10.1371/journal.pntd.0005949
Salah A. A., Robertson I., Mohamed A. (2015) Estimating the economic impact of Trypanosoma evansi infection on production of camel herds in Somaliland. Trop. Anim. Health Prod. 47: 707–714. https://doi.org/10.1007/s11250-015-0780-0
https://doi.org/10.1007/s11250-015-0780-0
Sculaccio S. A., Rodrigues E. M., Cordeiro A. T., et al. (2008) Selenocysteine incorporation in Kinetoplastid: Selenophosphate synthetase (SELD) from Leishmania major and Trypanosoma brucei. Mol. Biochem. Parasitol. 162: 165–171. https://doi.org/10.1016/j.molbiopara.2008.08.009
https://doi.org/10.1016/j.molbiopara.2008.08.009
Silva R. A. M., Seidl A., Ramirez L., Dávila A. M. R. (2002) Trypanosoma evansi e Trypanosoma vivax: Biologia Diagnóstico e Controle. Embrapa Pantanal, Corumbá, Mato Grosso, Brazil 141
Tuntasuvan D., Jarabrum W., Viseshakul N., et al. (2003) Chemotherapy of surra in horses and mules with diminazene aceturate. Vet. Parasitol. 110: 227–233.https://doi.org/10.1016/S0304-4017(02)00304-7
https://doi.org/10.1016/S0304-4017(02)00304-7
Ventura R. M., Takeda G. F., Silva R. A. M. S., et al. (2002) Genetic relatedness among Trypanosoma evansi stocks by random amplification of polymorphic DNA and evaluation of a synapomorphic DNA fragment for species-specific diagnosis.Int. J. Parasitol. 32: 53–63. https://doi.org/10.1016/S0020-7519(01)00314-9
https://doi.org/10.1016/S0020-7519(01)00314-9

Information

Information: Acta Protozoologica, 2021, pp. 21 - 29

Article type: Original research article

Authors

Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil

Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)

Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)

Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil

Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil

Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis. SC. Brazil

Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis. SC. Brazil

Instituto Federal de Santa Catarina (IFSC), Campus Lages, Brazil

Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)

https://orcid.org/0000-0001-5926-0286

Luiz Claudio Miletti
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
https://orcid.org/0000-0001-5926-0286 Orcid
All publications →

Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)

Published at: 05.08.2021

Article status: Open

Licence: CC BY  licence icon

Percentage share of authors:

Kaio Cesar Simiano Tavares (Author) - 12%
Maria Gabriela Casagrande Dambrós (Author) - 12%
André Saraiva Leão Antunes (Author) - 12%
Pietro Martin Danziato (Author) - 12%
Patricia Hermes Stoco (Author) - 12%
Aline Daiane Schlindwein (Author) - 12%
Renato Simões Moreira (Author) - 12%
Luiz Claudio Miletti (Author) - 16%

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