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Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein

Publication date: 05.08.2021

Acta Protozoologica, 2021, Volume 60, pp. 21 - 29

https://doi.org/10.4467/16890027AP.21.003.14063

Authors

,
Kaio Cesar Simiano Tavares
Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
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,
Maria Gabriela Casagrande Dambrós
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
All publications →
,
André Saraiva Leão Antunes
Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil
All publications →
,
Pietro Martin Danziato
Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil
All publications →
,
Patricia Hermes Stoco
Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis. SC. Brazil
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,
Aline Daiane Schlindwein
Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis. SC. Brazil
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,
Renato Simões Moreira
Instituto Federal de Santa Catarina (IFSC), Campus Lages, Brazil
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
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Luiz Claudio Miletti
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
https://orcid.org/0000-0001-5926-0286 Orcid
All publications →

Titles

Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein

Abstract

Selenoproteins have been described in all three domains of life and their function has been mainly associated with oxidative stress defense. Canonical elements required for selenoprotein production have been identified in members of the kinetoplastid group supporting the existence of a complete selenocysteine synthesis pathway in these organisms. Currently, nothing is known regarding the selenocysteine pathway in Trypanosoma evansi. In this study, we identified the expression of the elements selBselCselDPSTK and selTRYP at the mRNA level in T. evansi. All translated proteins (selD, PSTK, selTRYP and selB) have the domains predicted and higher identity with Trypanosoma brucei. gambiense. The selenophosphate synthetase protein was localized in the cytoplasm. Our results support the existence of an active selenocysteine pathway in T. evansi.

References

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Information

Information: Acta Protozoologica, 2021, Volume 60, pp. 21 - 29

Article type: Original article

Authors

Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil

Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)

Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)

Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil

Experimental Biology Center (NUBEX), Universidade de Fortaleza, Brazil

Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis. SC. Brazil

Laboratório de Protozoologia, Universidade Federal de Santa Catarina, Florianópolis. SC. Brazil

Instituto Federal de Santa Catarina (IFSC), Campus Lages, Brazil

Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)

https://orcid.org/0000-0001-5926-0286

Luiz Claudio Miletti
Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)
https://orcid.org/0000-0001-5926-0286 Orcid
All publications →

Laboratório de Hemoparasitas e Vetores Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC)

Published at: 05.08.2021

Article status: Open

Licence: CC BY  licence icon

Percentage share of authors:

Kaio Cesar Simiano Tavares (Author) - 12%
Maria Gabriela Casagrande Dambrós (Author) - 12%
André Saraiva Leão Antunes (Author) - 12%
Pietro Martin Danziato (Author) - 12%
Patricia Hermes Stoco (Author) - 12%
Aline Daiane Schlindwein (Author) - 12%
Renato Simões Moreira (Author) - 12%
Luiz Claudio Miletti (Author) - 16%

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