TY - JOUR
TI - Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein
AU - Tavares, Kaio Cesar Simiano
AU - Casagrande Dambrós, Maria Gabriela
AU - Antunes, André Saraiva Leão
AU - Danziato, Pietro Martin
AU - Stoco, Patricia Hermes
AU - Schlindwein, Aline Daiane
AU - Moreira, Renato Simões
AU - Miletti, Luiz Claudio
TI - Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein
AB - Selenoproteins have been described in all three domains of life and their function has been mainly associated with oxidative stress defense. Canonical elements required for selenoprotein production have been identified in members of the kinetoplastid group supporting the existence of a complete selenocysteine synthesis pathway in these organisms. Currently, nothing is known regarding the selenocysteine pathway in Trypanosoma evansi. In this study, we identified the expression of the elements selB, selC, selD, PSTK and selTRYP at the mRNA level in T. evansi. All translated proteins (selD, PSTK, selTRYP and selB) have the domains predicted and higher identity with Trypanosoma brucei. gambiense. The selenophosphate synthetase protein was localized in the cytoplasm. Our results support the existence of an active selenocysteine pathway in T. evansi.
VL - 2021
IS - Volume 60
PY - 2021
SN - 0065-1583
C1 - 1689-0027
SP - 21
EP - 29
DO - 10.4467/16890027AP.21.003.14063
UR - https://ejournals.eu/en/journal/acta-protozoologica/article/selenocysteine-in-trypanosoma-evansi-identification-of-the-genes-selb-selc-seld-pstk-seltryp-and-the-selenophosphate-synthetase-protein
KW - Trypanosoma evansi
KW - selenocysteine
KW - selB
KW - selC
KW - selD
KW - PSTK
KW - seltTRYP