Isolation of Thermotolerant Pathogenic Naegleria australiensis from Diverse Water Resources Including Household Drinking Water in Pakistan
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RIS BIB ENDNOTEIsolation of Thermotolerant Pathogenic Naegleria australiensis from Diverse Water Resources Including Household Drinking Water in Pakistan
Publication date: 14.12.2017
Acta Protozoologica, 2017, Volume 56, Issue 4, pp. 317 - 322
https://doi.org/10.4467/16890027AP.17.027.7829Authors
Isolation of Thermotolerant Pathogenic Naegleria australiensis from Diverse Water Resources Including Household Drinking Water in Pakistan
Naegleria is well recognized for primary amoebic meningoencephalitis which always results into death. To date there is not a single report demonstrating molecular identification of Naegleria from water resources of Pakistan thus the aim of the proposed study.Here, in total 135 various water samples (like domestic tap water, municipal water, sea water, well water, tube well water, canal water, boring water, sewage water, lake water and stream water) were collected across Pakistan and evaluated for Naegleria species. Naegleria australiensis (pathogenic) and Naegleria lovaniensis (non pathogenic) were isolated on non nutrient agar plates and were further identified by PCR and sequencing. To best of our knowledge we have described for the first time the isolation and molecular identification of thermotolerant pathogenic and non pathogenicNaegleria species from diverse water samples including drinking water across Pakistan. The presence of pathogenic Naegleria species in diverse water samples may add the health threat to the community.
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Information: Acta Protozoologica, 2017, Volume 56, Issue 4, pp. 317 - 322
Article type: Original article
University of Haripur, Haripur, Pakistan
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Saudia Arabia
Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Saudia Arabia
Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Saudia Arabia
College of Arts and Sciences, Department of Biological Sciences and Chemistry, University of Nizwa, Birkat Al-Mouz, Nizwa, 616, Sultanate of Oman
Faculty of Natural and Health Sciences, Department of Biological Science, University of Baltistan, Skardu, Gilgit-Baltistan, 16100, Pakistan
Published at: 14.12.2017
Article status: Open
Licence: CC BY-NC-ND
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