TY - JOUR
TI - Isolation of Thermotolerant Pathogenic Naegleria australiensis from Diverse Water Resources Including Household Drinking Water in Pakistan
AU - Tanveer, Tania
AU - Hameed, Abdul
AU - Bin-Dukhyil, Abdul-Aziz A.
AU - Alaidarous, Mohammed
AU - Matin, Abdul
TI - Isolation of Thermotolerant Pathogenic Naegleria australiensis from Diverse Water Resources Including Household Drinking Water in Pakistan
AB - Naegleria is well recognized for primary amoebic meningoencephalitis which always results into death. To date there is not a single report demonstrating molecular identification of Naegleria from water resources of Pakistan thus the aim of the proposed study.Here, in total 135 various water samples (like domestic tap water, municipal water, sea water, well water, tube well water, canal water, boring water, sewage water, lake water and stream water) were collected across Pakistan and evaluated for Naegleria species. Naegleria australiensis (pathogenic) and Naegleria lovaniensis (non pathogenic) were isolated on non nutrient agar plates and were further identified by PCR and sequencing. To best of our knowledge we have described for the first time the isolation and molecular identification of thermotolerant pathogenic and non pathogenicNaegleria species from diverse water samples including drinking water across Pakistan. The presence of pathogenic Naegleria species in diverse water samples may add the health threat to the community.
VL - 2017
IS - Volume 56, Issue 4
PY - 2017
SN - 0065-1583
C1 - 1689-0027
SP - 317
EP - 322
DO - 10.4467/16890027AP.17.027.7829
UR - https://ejournals.eu/en/journal/acta-protozoologica/article/isolation-of-thermotolerant-pathogenic-naegleria-australiensis-from-diverse-water-resources-including-household-drinking-water-in-pakistan
KW - Thermotolerant pathogenic Naegleria
KW - Primary Amoebic Meningoencephalitis
KW - Isolation
KW - Molecular identification
KW - Pakistan