Concentrations of volatile substances in costal cartilage in relation to blood and urine – preliminary studies
cytuj
pobierz pliki
RIS BIB ENDNOTEWybierz format
RIS BIB ENDNOTEConcentrations of volatile substances in costal cartilage in relation to blood and urine – preliminary studies
Data publikacji: 01.10.2021
Archiwum Medycyny Sądowej i Kryminologii, 2021, Vol. 71 (1-2), s. 38 - 46
https://doi.org/10.5114/amsik.2021.106014Autorzy
Concentrations of volatile substances in costal cartilage in relation to blood and urine – preliminary studies
Aim: The study aimed to examine whether volatile substances (ethanol, isopropanol, and acetone) can be detected in costal cartilage and also if concentrations of detected substances reliably reflect their concentrations in the peripheral blood – the standard forensic material for toxicological analyses. Such knowledge can be useful in cases when a cadaver’s blood is unavailable or contaminated.
Material and methods: Ethanol, isopropanol, and acetone concentrations were determined in samples of unground costal cartilage (UCC), ground costal cartilage (GCC), femoral venous blood, and urine. The samples were analysed by gas chromatography (GC) with a flame ionization detector using headspace analysis.
Results: Volatile substances were detected in 12 out of 100 analysed samples. There was a strong positive correlation between ethanol concentration in the blood and urine (r = 0.899, p < 0.001), UCC (r = 0.809, p < 0.01), and GCC (r = 0.749, p < 0.01). A similar strong correlation was found for isopropanol concentration in the blood and urine (r = 0.979, p < 0.001), UCC (r = 0.866, p < 0.001), and GCC (r = 0.942, p < 0.001). Acetone concentration in the blood strongly correlated only with its concentration in urine (r = 0.960, p < 0.001).
Conclusions: We demonstrated for the first time the possibility of detecting volatile substances: ethanol, isopropanol and acetone in a human costal cartilage. Also, the study showed that higher volatiles concentrations were better determined in ground samples.
1. Borowska-Solonynko A, Siwińska-Ziółkowska A, Piotrkowicz M, Wysmołek M, Demkow M. Analysis of the origin and importance of acetone and isopropanol levels in the blood of the deceased for medico-legal testimony. Arch Med Sadowej Kryminol 2014; 64: 230-234.
2. Boumba VA, Ziavrou KS, Vougiouklakis T. Biochemical pathways generating post-mortem volatile compounds co-detected during forensic ethanol analyses. Forensic Sci Int 2008; 174: 133-151.
3. Wille SM, Lambert WE. Volatile substance abuse-post-mortem diagnosis. Forensic Sci Int 2004; 142: 135-156.
4. Vujasinovic M, Kocar M, Kramer K, Bunc M, Brvar M. Poisoning with 1-propanol and 2-propanol. Hum Exp Toxicol 2007; 26: 975-978.
5. Palmiere C. Postmortem diagnosis of diabetes mellitus and its complications. Croat Med J 2015; 56: 181-193.
6. Jones AE, Summers RL. Detection of isopropyl alcohol in a patient with diabetic ketoacidosis. J Emerg Med 2000; 19: 165-168.
7. Palmiere C, Bardy D, Letovanec I, et al. Biochemical markers of fatal hypothermia. Forensic Sci Int 2013; 226: 54-61.
8. Palmiere C, Mangin P. Postmortem biochemical investigations in hypothermia fatalities. Int J Legal Med 2013; 127: 267-276.
9. Palmiere C, Tettamanti C, Augsburger M, et al. Postmortem biochemistry in suspected starvation-induced ketoacidosis. J Forensic Leg Med 2016; 42: 51-55.
10. Davis PL, Dal Cortivo LA, Maturo J. Endogenous isopropanol: forensic and biochemical implications. J Anal Toxicol 1984; 8: 209-212.
11. Dwyer JB, Tamama K. Ketoacidosis and trace amounts of isopropanol in a chronic alcoholic patient. Clin Chim Acta 2013; 415: 245-249.
12. Chan KM, Wong ET, Matthews WS. Severe isopropanolemia without acetonemia or clinical manifestations of isopropanol intoxication. Clin Chem 1993; 39: 1922-1925.
13. Molina DK. A characterization of sources of isopropanol detected on postmortem toxicologic analysis. J Forensic Sci 2010; 55: 998-1002.
14. Wu X, Lu G, Qi B, Wang R, Guo D, Liu X. Antifreeze poisoning: a case report. Exp Ther Med 2017; 13: 701-704.
15. Slaughter RJ, Mason RW, Beasley DM, Vale JA, Schep LJ. Isopropanol poisoning. Clin Toxicol (Phila.) 2014; 52: 470-478.
16. Bévalot F, Cartiser N, Bottinelli C, Fanton L, Guitton J. Vitreous humor analysis for the detection of xenobiotics in forensic toxicology: a review. Forensic Toxicol 2016; 34: 12-40.
17. Tominaga M, Ishikawa T, Michiue T, et al. Postmortem analyses of gaseous and volatile substances in pericardial fluid and bone marrow aspirate. J Anal Toxicol 2013; 37: 147-151.
18. Lewis RJ, Johnson RD, Angier MK, Vu NT. Ethanol formation in unadulterated postmortem tissues. Forensic Sci Int 2004; 146: 17-24.
19. Jenkins AJ, Levine BS, Smialek JE. Distribution of ethanol in postmortem liver. J Forensic Sci 1995; 40: 611-613.
20. Garriott JC. Skeletal muscle as an alternative specimen for alcohol and drug analysis. J Forensic Sci 1991; 36: 60-69.
21. Chun HJ, Poklis JL, Poklis A, Wolf CE. Development and validation of a method for alcohol analysis in brain tissue by headspace gas chromatography with flame ionization detector. J Anal Toxicol 2016; 40: 653-658.
22. Boonyoung S, Narongchai P, Junkuy A. The relationship of alcohol concentration in epidural or acute subdural hematoma compared with vitreous humor and femoral blood. J Med Assoc Thai 2008; 91: 754-758.
23. Huwe LW, Brown WE, Hu JC, Athanasiou KA. Characterization of costal cartilage and its suitability as a cell source for articular cartilage tissue engineering. J Tissue Eng Regen Med 2018; 12: 1163-1176.
24. Stacey MW. Biochemical and histological differences between costal and articular cartilages. In: Saxena AK (ed.). Chest wall deformities. Springer, London 2017, 81-99.
25. Grogan SP, Chen X, Sovani S, et al. Influence of cartilage extracellular matrix molecules on cell phenotype and neocartilage formation. Tissue Eng Part A 2014; 20: 264-274.
26. Yotsuyanagi T, Yamashita K, Yamauchi M, et al. Establishment of a standardized technique for concha-type microtia-how to incorporate the cartilage frame into the remnant ear. Plast Reconstr Surg Glob Open 2019; 26: e2337.
27. Mao X, Li X, Jia J, et al. Validity and reliability of three-dimensional costal cartilage imaging for donor-site assessment and clinical application in microtia reconstruction patients: A prospective study of 22 cases. Clin Otolaryngol 2020; 45: 204-210.
28. Zhang L, Ma WS, Bai JP, Li XX, Li HD, Zhu T. Comprehensive application of autologous costal cartilage grafts in rhino- and mentoplasty. J Craniofac Surg 2019; 30: 2174-2177.
29. Mohan R, Shanmuga Krishnan RR, Rohrich RJ. Role of fresh frozen cartilage in revision rhinoplasty. Plast Reconstr Surg 2019; 144: 614-622.
30. Talaat WM, Ghoneim MM, El-Shikh YM, Elkashty SI, Ismail MAG, Keshk TFA. Anthropometric analysis of secondary cleft lip rhinoplasty using costal cartilage graft. J Craniofac Surg 2019; 30: 2464-2468.
31. Saadi R, Loloi J, Schaefer E, Lighthall JG. Outcomes of cadaveric allograft versus autologous cartilage graft in functional septorhinoplasty. Otolaryngol Head Neck Surg 2019; 161: 779-786.
32. Dinis-Oliveira RJ, Vieira, DN, Magalhães, T. Guidelines for collection of biological samples for clinical and forensic toxicological analysis. Forensic Sci Res 2017; 1: 42-51.
33. Tomsia M, Nowicka J, Skowronek R, et al. A comparative study of ethanol concentration in costal cartilage in relation to blood and urine. Processes 2020; 8: 1637.
34. Nowicka J, Kulikowska J, Chowaniec C, et al. Medicolegal and toxicological aspects of isopropanol levels in post-mortem material. Problems Forensic Sci 2010; 82: 191-199.
35. Drela E, Rosół M, Trnka J. Poisonings with iso-propanol and acetone as the substitutes of ethyl alcohol. Problems Forensic Sci 2004; 58: 58-69.
36. Zhang K, Fan F, Tu M, et al. The role of multislice computed tomography of the costal cartilage in adult age estimation. Int J Legal Med 2018; 132: 791-798.
37. Ikeda T. Estimating age at death based on costal cartilage calcification. Tohoku J Exp Med 2017; 243: 237-246.
38. Meng H, Zhang M, Xiao B, Chen X, Yan J, Zhao Z. Forensic age estimation based on the pigmentation in the costal cartilage from human mortal remains. Leg Med (Tokyo) 2019; 40: 32-36.
39. Alibegović A, Blagus R, Martinez IZ. Safranin O without fast green is the best staining method for testing the degradation of macromolecules in a cartilage extracellular matrix for the determination of the postmortem interval. Forensic Sci Med Pathol 2020; 16: 252-258.
40. Siriboonpiputtana T, Rinthachai T, Shotivaranon J, Peonim V, Rerkamnuaychoke B. Forensic genetic analysis of bone remain samples. Forensic Sci Int 2018; 284: 167-175.
41. Redouane F, Lambert M, De Greef J, Malghem J, Lecouvet FE. Primary infectious costochondritis due to Prevotella nigrescens in an immunocompetent patient: clinical and imaging findings. Skeletal Radiol 2019; 48: 1305-1309.
Informacje: Archiwum Medycyny Sądowej i Kryminologii, 2021, Vol. 71 (1-2), s. 38 - 46
Typ artykułu: Oryginalny artykuł naukowy
Department of Forensic Medicine and Forensic Toxicology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, Katowice, Poland
Department of Physical Sciences and Forensic Science Programs, Alabama State University, Montgomery, AL, USA
Katedra i Zakład Medycyny Sądowej i Toksykologii Sądowo-Lekarskiej, Wydział Nauk Medycznych w Katowicach,
Śląski Uniwersytet Medyczny w Katowicach
Polska
Department of Physical Sciences and Forensic Science Programs, Alabama State University, Montgomery, AL, USA
Department of Statistics, Department of Instrumental Analysis, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Katowice, Poland
Publikacja: 01.10.2021
Otrzymano: 15.01.2021
Zaakceptowano: 04.03.2021
Status artykułu: Otwarte
Licencja: CC-BY-NC-SA
Udział procentowy autorów:
Korekty artykułu:
-Języki publikacji:
AngielskiLiczba wyświetleń: 346
Liczba pobrań: 350