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Acta Protozoologica

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The function of ciliopathy protein FOP on cilia and cortical microtubule cytoskeleton in Euplotes amieti

Publication date: 2023

Acta Protozoologica, 2023, Volume 62, pp. 45 - 56

https://doi.org/10.4467/16890027AP.23.005.18868

Authors

,
Junlin WU
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China
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,
Jiaqi Yin
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China
All publications →
,
Zixiang Xu
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China
All publications →
,
Yingli Liu
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China
All publications →
,
Huanyong Qin
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China
All publications →
Xin Sheng
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China; School of Life Science, East China Normal University, Shanghai, China
All publications →

Abstract

FOP is a centriole satellite protein involved in ciliogenesis. Although centriole satellites are involved in centrosome and ciliumrelated protein trafficking, their functions related to ciliary assembly and maintenance of ciliary microtubule stability remain unclear. In this study, the function of the FOP gene in Euplotes amieti was investigated by interfering with its expression using RNAi. As a result, expression levels of the ciliary assembly-related proteins BBS8 and IFT88 were down-regulated. Swimming speeds also decreased and the Euplotes were only able to spin in circles, which suggested that the FOP protein is an important protein involved in ciliary motion. Further observations of Euplotes amieti microstructure and ultrastructure via immunofluorescence and transmission electron microscopy revealed that FOP not only participated in the formation of the ventral ciliary basal body but also played an important role in the maintenance of cortical microtubules, which is fundamental for the morphological structure of Euplotes amieti.

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Information

Information: Acta Protozoologica, 2023, pp. 45 - 56

Article type: Original research article

Authors

Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China

Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China

Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China

Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China

Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China

Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China; School of Life Science, East China Normal University, Shanghai, China

Published at: 2023

Article status: Open

Licence: CC BY  licence icon

Percentage share of authors:

Junlin WU (Author) - 16.66%
Jiaqi Yin (Author) - 16.66%
Zixiang Xu (Author) - 16.66%
Yingli Liu (Author) - 16.66%
Huanyong Qin (Author) - 16.66%
Xin Sheng (Author) - 16.66%

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Publication languages:

English

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