The function of ciliopathy protein FOP on cilia and cortical microtubule cytoskeleton in Euplotes amieti
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RIS BIB ENDNOTEThe function of ciliopathy protein FOP on cilia and cortical microtubule cytoskeleton in Euplotes amieti
Publication date: 18.04.2024
Acta Protozoologica, 2023, Volume 62, pp. 45 - 56
https://doi.org/10.4467/16890027AP.23.005.18868Authors
The function of ciliopathy protein FOP on cilia and cortical microtubule cytoskeleton in Euplotes amieti
FOP is a centriole satellite protein involved in ciliogenesis. Although centriole satellites are involved in centrosome and ciliumrelated protein trafficking, their functions related to ciliary assembly and maintenance of ciliary microtubule stability remain unclear. In this study, the function of the FOP gene in Euplotes amieti was investigated by interfering with its expression using RNAi. As a result, expression levels of the ciliary assembly-related proteins BBS8 and IFT88 were down-regulated. Swimming speeds also decreased and the Euplotes were only able to spin in circles, which suggested that the FOP protein is an important protein involved in ciliary motion. Further observations of Euplotes amieti microstructure and ultrastructure via immunofluorescence and transmission electron microscopy revealed that FOP not only participated in the formation of the ventral ciliary basal body but also played an important role in the maintenance of cortical microtubules, which is fundamental for the morphological structure of Euplotes amieti.
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Information: Acta Protozoologica, 2023, Volume 62, pp. 45 - 56
Article type: Original article
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China
Department of Biochemistry, Zunyi Medical University, Zunyi, Guizhou, China; School of Life Science, East China Normal University, Shanghai, China
Published at: 18.04.2024
Received at: 03.08.2023
Accepted at: 06.11.2023
Article status: Open
Licence: CC BY
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