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Gamma Radiation Tolerance and Protein Carbonylation Caused by Irradiation of Resting Cysts in the Free-living Ciliated Protist Colpoda cucullus

Publication date: 2020

Acta Protozoologica, 2020, Volume 59, Issue 2, pp. 67 - 75

https://doi.org/10.4467/16890027AP.20.006.12674

Authors

,
Ryota Saito
Department of Chemistry and Biotechnology, Kochi University, Kochi 780-8520, Japan
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
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,
Ryota Koizumi
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
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,
Tatsuya Sakai
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
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,
Taiga Shimizu
Department of Chemistry and Biotechnology, Kochi University, Kochi 780-8520, Japan
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
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,
Taiki Ono
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
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Yoichiro Sogame
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
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Titles

Gamma Radiation Tolerance and Protein Carbonylation Caused by Irradiation of Resting Cysts in the Free-living Ciliated Protist Colpoda cucullus

Abstract

The ciliate Colpoda cucullus forms resting cysts to survive unfavorable environmental stresses. In this study, we have shown that Colpoda resting cysts survived exposure to a gamma radiation dose of 4000 Gy, although vegetative cells were killed by 500 Gy. After 4000 Gy irradiation, more than 90% of resting cysts and approximately 70% of dry cysts could excyst to form vegetative cells. In both cases, the excystment gradually increased after the induction of excystment. In addition, we also showed that protein carbonylation level was increased by gamma irradiation, but decreased by incubation in the cyst state. These results indicated that cell damage was repaired in resting cysts. Colpoda probably developed tolerance to gamma radiation by forming resting cysts as a strategy for growth in terrestrial environments, as part of contending with the stress due to reactive oxygen species caused by desiccation.

References

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Information

Information: Acta Protozoologica, 2020, Volume 59, Issue 2, pp. 67 - 75

Article type: Original article

Authors

Department of Chemistry and Biotechnology, Kochi University, Kochi 780-8520, Japan

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

Department of Chemistry and Biotechnology, Kochi University, Kochi 780-8520, Japan

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

Published at: 2020

Received at: 10.01.2020

Accepted at: 22.05.2020

Article status: Open

Licence: CC BY-NC-ND  licence icon

Percentage share of authors:

Ryota Saito (Author) - 16%
Ryota Koizumi (Author) - 16%
Tatsuya Sakai (Author) - 16%
Taiga Shimizu (Author) - 16%
Taiki Ono (Author) - 16%
Yoichiro Sogame (Author) - 20%

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