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First Molecular Detection of Giardia duodenalis Assemblage B in a Free-Living European Wildcat (Felis s. silvestris) from Luxembourg

Publication date: 25.07.2019

Acta Protozoologica, 2019, Volume 58, Issue 1, pp. 1 - 5

https://doi.org/10.4467/16890027AP.19.001.10832

Authors

,
Piotr Solarczyk
Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Poznan, Poland
https://orcid.org/0000-0002-2862-4742 Orcid
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,
Natalia Osten-Sacken
Fondation Fauna-Flora, Luxembourg
Centre for Veterinary Sciences, Nicolaus Copernicus University, Toruń, Poland
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,
Alain C. Frantz
Musée National d‘Histoire Naturelle, 2160 Luxembourg
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,
Simone Schneider
Biological Station SICONA, Olm, Luxembourg
Musée National d‘Histoire Naturelle, 2160 Luxembourg
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,
Jacques B. Pir
Musée National d‘Histoire Naturelle, 2160 Luxembourg
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Mike Heddergott
Musée National d‘Histoire Naturelle, 2160 Luxembourg
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Abstract

Giardia duodenalis is one of the most widespread intestinal parasites of humans and other vertebrates. In terms of public health, identification of Giardia assemblages in wildlife is important because only some assemblages of G. duodenalis can infect humans. Here, we use loop-mediated isothermal amplification (LAMP) and  genotyping of analysis of the β-giardin gene to screen the zoonotic assemblages of G. duodenalis recovered from faeces of free-living European wildcats (Felis s. silvestris) from Luxembourg. Giardia DNA was detected in one animal (10%) and assigned to assemblage B by both methods. This is the first detection and genotyping  of G. duodenalis in a European wild felid in general, and of assemblage B in particular. Free-living wildcats may act as reservoirs of G. duodenalis infectious for humans and other wildlife and domestic animals. Using a combination of LAMP- and genotyping-based methods allowed effective, sensitive, and rapid detection of a zoonotic G. duodenalis assemblage B in wildlife.

Received on 8th April, 2019; revised on 10th May, 2019; accepted on 19th June, 2019

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Information

Information: Acta Protozoologica, 2019, pp. 1 - 5

Article type: Original research article

Authors

https://orcid.org/0000-0002-2862-4742

Piotr Solarczyk
Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Poznan, Poland
https://orcid.org/0000-0002-2862-4742 Orcid
All publications →

Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Poznan, Poland

Fondation Fauna-Flora, Luxembourg

Centre for Veterinary Sciences, Nicolaus Copernicus University, Toruń, Poland

Musée National d‘Histoire Naturelle, 2160 Luxembourg

Biological Station SICONA, Olm, Luxembourg

Musée National d‘Histoire Naturelle, 2160 Luxembourg

Musée National d‘Histoire Naturelle, 2160 Luxembourg

Musée National d‘Histoire Naturelle, 2160 Luxembourg

Published at: 25.07.2019

Article status: Open

Licence: CC BY-NC-ND  licence icon

Percentage share of authors:

Piotr Solarczyk (Author) - 16%
Natalia Osten-Sacken (Author) - 16%
Alain C. Frantz (Author) - 16%
Simone Schneider (Author) - 16%
Jacques B. Pir (Author) - 16%
Mike Heddergott (Author) - 20%

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English

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