First Molecular Detection of Giardia duodenalis Assemblage B in a Free-Living European Wildcat (Felis s. silvestris) from Luxembourg
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RIS BIB ENDNOTEFirst Molecular Detection of Giardia duodenalis Assemblage B in a Free-Living European Wildcat (Felis s. silvestris) from Luxembourg
Publication date: 25.07.2019
Acta Protozoologica, 2019, Volume 58, Issue 1, pp. 1 - 5
https://doi.org/10.4467/16890027AP.19.001.10832Authors
First Molecular Detection of Giardia duodenalis Assemblage B in a Free-Living European Wildcat (Felis s. silvestris) from Luxembourg
Giardia duodenalis is one of the most widespread intestinal parasites of humans and other vertebrates. In terms of public health, identification of Giardia assemblages in wildlife is important because only some assemblages of G. duodenalis can infect humans. Here, we use loop-mediated isothermal amplification (LAMP) and genotyping of analysis of the β-giardin gene to screen the zoonotic assemblages of G. duodenalis recovered from faeces of free-living European wildcats (Felis s. silvestris) from Luxembourg. Giardia DNA was detected in one animal (10%) and assigned to assemblage B by both methods. This is the first detection and genotyping of G. duodenalis in a European wild felid in general, and of assemblage B in particular. Free-living wildcats may act as reservoirs of G. duodenalis infectious for humans and other wildlife and domestic animals. Using a combination of LAMP- and genotyping-based methods allowed effective, sensitive, and rapid detection of a zoonotic G. duodenalis assemblage B in wildlife.
Received on 8th April, 2019; revised on 10th May, 2019; accepted on 19th June, 2019
Ansorge H. (1995) Notizen zur Altersbestimmung nach Wachstumslinien an Säugetierschädeln. In: Methoden feldökol. Säugetierforsch. Wiss Beitr Univ Halle, (Eds. M. Stubbe , A. Stubbe , D. Heidecke ) Halle, 95–102
Beck R., Sprong H., Bata I., Lucinger S., Pozio E., Cacciò S. M. (2011) Prevalence and molecular typing of Giardia spp. in captive mammals at the zoo of Zagreb, Croatia. Vet Parasitol 175(1–2): 40–46
Burnet J. B. Ogorzaly L., Penny C., Cauchie H. M. (2015) Fine-Scale spatial heterogeneity in the distribution of waterborne protozoa in a drinking water reservoir. Int J Environ Res Public Health 12(9): 11910–1128
Burnet J. B., Penny C., Ogorzaly L., Cauchie H. M. (2014) Spatial and temporal distribution of Cryptosporidium and Giardia in a drinking water resource: implications for monitoring and risk assessment. Sci Total Environ 472: 1023–1035
Cacciò S. M., Beck R., Lalle M., Marinculic A., Pozio E. (2008) Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B. Int J Parasitol 38(13): 1523–1531
Cacciò S. M., Beck R., Almeida A., Bajer A., Pozio E. (2010) Identification of Giardia species and Giardia duodenalis assemblages by sequence analysis of the 5.8S rDNA gene and internal transcribed spacers. Parasitology 137(6): 919–925
Cacciò S. M., De Giacomo M., Pozio E. (2002) Sequence analysis of the beta-giardin gene and development of a polymerase chain reaction restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int J Parasitol 32(8): 1023–1030
Cacciò S. M., Lalle M., Svärd S. G. (2018) Host specificity in the Giardia duodenalis species complex. Infect Gent Evol 66: 335–345
de Lucio A., Bailo B., Aguilera M., Cardona G. A., Fernández-Crespo J. C., Carmena D. (2017) No molecular epidemiological evidence supporting household transmission of zoonotic Giardia duodenalis and Cryptosporidium spp. from pet dogs and cats in the province of Álava, Northern Spain. Acta Trop 170: 48–56
Helmi K., Skraber S., Burnet J. B., Leblanc L., Hoffmann L., Cauchie H. M. (2011) Two-year monitoring of Cryptosporidium parvum and Giardia lamblia occurrence in a recreational and drinking water reservoir using standard microscopic and molecular biology techniques. Environ Monit Assess 179(1–4): 163–175
Jaros D., Zygner W., Jaros S., Wedrychowicz W. (2011) Detection of Giardia intestinalis assemblages A, B and D in domestic cats from Warsaw, Poland. Pol J Microbiol 60(3): 259–263
Feng Y., Xiao L. (2011) Zoonotic potential and molecular epidemiology of Giardia species and Giardiasis. Clin Microbiol Rev 24(1): 110–140
Gil H., Cano L., de Lucio A., Bailo B., de Mingo M. H., Cardona G. A., Fernández-Basterra J. A., Aramburu-Aguirre J., López- Molina N., Carmena D. (2017) Detection and molecular diversity of Giardia duodenalis and Cryptosporidium spp. in sheltered dogs and cats in Northern Spain. Infect Genet Evol 50: 62–69
Li J., Wang H., Wang R., Zhang L. (2017) Giardia duodenalis infections in humans and other animals in China. Front Microbiol 8: 2004
Liu H., Shen Y., Liu A., Yin J., Yuan Z., Jiang Y., Pan W., Zhang Y., Zhao W., Cao J. (2017) Occurrence and multilocus genotyping of Giardia duodenalis in pets and zoo animals in Shanghai, China. The Journal of Infection in Developing Countries 11(6): 479–486
Kumar S., Stecher G., Tamura K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol 33(7): 1870–1874
Kváč M., Hofmannová L., Ortega Y., Holubová N., Horčičková M., Kicia M., Hlásková L., Květoňová D., Sak B., McEvoy J. (2017) Stray cats are more frequently infected with zoonotic protists than pet cats. Folia Parasitol (Praha) 64: 034
Lebbad M., Mattsson J. G., Christensson B., Ljungström B., Backhans A., Andersson J. O., Svärd S. G. (2010) From mouse to moose: Multilocus genotyping of Giardia isolates from various animal species. Vet Parasitol 168(3-4): 231–239
McDowall R. M., Peregrine A. S., Leonard E. K., Lacombe C., Lake M., Rebelo A. R., Cai H. Y. (2011) Evaluation of the zoonotic potential of Giardia duodenalis in fecal samples from dogs and cats in Ontario. Can Vet J 52(12): 1329–1333
Oates S. C., Miller M. A., Hardin D., Conrad P. A., Melli A., Jessup D. A., Dominik C., Roug A., Tinker M.T., Miller W. A. (2012) Prevalence, environmental loading, and molecular characterization of Cryptosporidium and Giardia isolates from domestic and wild animals along the Central California Coast. Appl Environ Microbiol 78(24): 8762–8772
Palmer C. S., Traub R. J., Robertson I. D., Devlin G., Rees R., Thompson R. C. (2008) Determining the zoonotic significance of Giardia and Cryptosporidium in Australian dogs and cats. Vet Parasitol 154(1-2): 142–147
Pallant L., Barutzki D., Schaper R., Thompson R. C. A. (2015) The epidemiology of infections with Giardia species and genotypes in well cared for dogs and cats in Germany. Parasit Vectors 8: 2
Piechocki R., Stiefel A. (1988) Über die Altersstruktur der Verluste der Wildkatze (Felis s. silvestris Schreber 1777). Hercynia N F 25: 235–258
Plutzer J., Karanis P. (2009) Rapid identification of Giardia duodenalis by loop-mediated isothermal amplification (LAMP) from faecal and environmental samples and comparative findings by PCR and real-time PCR methods. Parasitol Res 104(6): 1527–1533
Read C. M., Monis P. T., Thompson R. C. (2004) Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP. Infect Genet Evol 4(2): 125–130
Steyer K., Kraus R. H. S., Mölich T., Anders O., Cocchiararo B., Frosch C., Geib A., Götz M., Herrmann M., Hupe K., Kohnen A., Krüger M., Müller F., Pir J. P., Reiners T. E., Roch S., Schade U., Schiefenhövel P., Siemund M., Simon O., Steeb S., Streif S., Streit B., Thein .J, Tiesmeyer A., Trinzen M., Vogel B., Nowak C. (2016) Large-scale genetic census of an elusive carnivore, the European wildcat (Felis s. silvestris). Conserv Genet 17(5): 1183–1199
Sotiriadou I., Pantchev N., Gassmann D., Karanis P. (2013) Molecular identification of Giardia and Cryptosporidium from dogs and cats. Parasite 2013:20:8
Souza S. L., Gennari S. M., Richtzenhain L. J., Pena H. F., Funada M. R., Cortez A., Gregori F., Soares R. M. (2007) Molecular identification of Giardia duodenalis isolates from humans, dogs, cats and cattle from the state of São Paulo, Brazil, by sequence analysis of fragments of glutamate dehydrogenase (gdh) coding gene. Vet Parasitol 149(3–4): 258–264
Suzuki J., Murata R., Kobayashi .S, Sadamasu K., Kai A., Takeuchi T. (2011) Risk of human infection with Giardia duodenalis from cats in Japan and genotyping of the isolates to assess the route of infection in cats. Parasitology 138(4): 493–500
Information: Acta Protozoologica, 2019, Volume 58, Issue 1, pp. 1 - 5
Article type: Original article
Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Poznan, Poland
Fondation Fauna-Flora, Luxembourg
Centre for Veterinary Sciences, Nicolaus Copernicus University, Toruń, Poland
Musée National d‘Histoire Naturelle, 2160 Luxembourg
Biological Station SICONA, Olm, Luxembourg
Musée National d‘Histoire Naturelle, 2160 Luxembourg
Musée National d‘Histoire Naturelle, 2160 Luxembourg
Musée National d‘Histoire Naturelle, 2160 Luxembourg
Published at: 25.07.2019
Article status: Open
Licence: CC BY-NC-ND
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