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Ultrastructure and Phylogeny of Pleistophora beebei sp. nov. (Microsporidia) Infecting the Amazonian Teleostean Brachyhypopomus beebei (fam. Hypopomidae)

Publication date: 19.12.2016

Acta Protozoologica, 2016, Volume 55, Issue 4, pp. 259 - 268

https://doi.org/10.4467/16890027AP.16.022.6009

Authors

,
Graça Casal
Laboratory of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, Portugal
Department of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), and Laboratory of Pathology, Centre for Marine and Environmental Research (CIIMAR/UP), University of Porto, Portugal; Departmento de Ciências, Instituto Superior de Ciências da Saúde, Gandra, Portugal;
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,
Edilson Rodrigues Matos
Carlos Azevedo Research Laboratory, Institute of Animal Health and Production, Federal Rural University of Amazonia, Belém, PA, Brazil
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,
Sónia Rocha
Laboratory of Animal Pathology, Interdisciplinary Centre for Marine Environmental Research (CIIMAR/UP), University of Porto, Porto, Portugal; Laboratory of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, Portugal
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,
José Sindeaux Neto
Carlos Azevedo Research Laboratory, Federal Rural University of Amazonia, Belém (Pará), Brazil
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,
Saleh Al-Quraishy
Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia
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Carlos Azevedo
King Saud University, Riyadh, Saudi Arabia
Department of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), and Laboratory of Pathology, Centre for Marine and Environmental Research (CIIMAR/UP), University of Porto, Portugal; Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia;
All publications →

Titles

Ultrastructure and Phylogeny of Pleistophora beebei sp. nov. (Microsporidia) Infecting the Amazonian Teleostean Brachyhypopomus beebei (fam. Hypopomidae)

Abstract

A new microsporidian, Pleistophora beebei sp. nov., parasitizing the freshwater benthopelagic teleostean fish Brachyhypopomus beebei Schultz, 1944 (fam. Hypopomidae) collected from the Amazon River is described based on molecular and morphological studies. The parasite develops in the skeletal muscle of the abdominal cavity, forming a whitish cyst-like containing several groups of two types of spores (macrospores and microspores), which were observed in close contact with the myofibrils. Small groups of macrospores (ovoid elongate, tapering more anteriorly than posteriorly and measuring about 7.8 ± 0.4 × 4.7 ± 0.2 µm) were observed among the numerous microspores (lightly pyriform to ellipsoidal with rounded ends, measured about 4.7 ± 0.3 × 2.8 ± 0.4 µm). Both types of spores possessed a single large posterior vacuole containing flocculent material. The ultrastructural aspects observed, together with the formation of a cyst-like, suggest that the parasite belongs to the genus Pleistophora. This taxonomic positioning was confirmed by the molecular analysis of the SSU rRNA gene and Maximum-likelihood (ML) inference. Comparison to similar species previously described, recognized this as a new species, herein named Pleistophora beebei sp. nov.

References

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Information

Information: Acta Protozoologica, 2016, Volume 55, Issue 4, pp. 259 - 268

Article type: Original article

Authors

Laboratory of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, Portugal

Department of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), and Laboratory of Pathology, Centre for Marine and Environmental Research (CIIMAR/UP), University of Porto, Portugal; Departmento de Ciências, Instituto Superior de Ciências da Saúde, Gandra, Portugal;

Carlos Azevedo Research Laboratory, Institute of Animal Health and Production, Federal Rural University of Amazonia, Belém, PA, Brazil

Laboratory of Animal Pathology, Interdisciplinary Centre for Marine Environmental Research (CIIMAR/UP), University of Porto, Porto, Portugal; Laboratory of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, Portugal

Carlos Azevedo Research Laboratory, Federal Rural University of Amazonia, Belém (Pará), Brazil

Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia

King Saud University, Riyadh, Saudi Arabia

Department of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), and Laboratory of Pathology, Centre for Marine and Environmental Research (CIIMAR/UP), University of Porto, Portugal; Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia;

Published at: 19.12.2016

Article status: Open

Licence: None

Percentage share of authors:

Graça Casal (Author) - 16%
Edilson Rodrigues Matos (Author) - 16%
Sónia Rocha (Author) - 16%
José Sindeaux Neto (Author) - 16%
Saleh Al-Quraishy (Author) - 16%
Carlos Azevedo (Author) - 20%

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English