Tolerance of Colpoda cucullus Nag-1 Resting Cysts and Presumed Structure for Protection against UV Light
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RIS BIB ENDNOTETolerance of Colpoda cucullus Nag-1 Resting Cysts and Presumed Structure for Protection against UV Light
Publication date: 2020
Acta Protozoologica, 2020, Volume 59, Issue 1, pp. 55 - 60
https://doi.org/10.4467/16890027AP.20.004.12160Authors
Tolerance of Colpoda cucullus Nag-1 Resting Cysts and Presumed Structure for Protection against UV Light
Resting cysts of the terrestrial ciliate Colpoda cucullus (Nag-1 strain) are highly resistant to UV light. It has been speculated that auto-fluorescent (blue fluorescent) particles surrounding the nuclei and yellowish fluorescent layers of the cyst wall are the candidate structures for the protection of the cellular components from UV light. The UV resistance of encysting cells was quickly acquired up to 5 h after the onset of encystment induction, and then gradually increased for several days. The less fluorescent ectocyst layer, yellowish fluorescent first-synthesized endocyst layer (en-1) and the NSPs were formed within 5 h after the onset of encystment induction, and thereafter endocyst layers became gradually thicker for several days. The cyst wall sample (ectocyst and endocyst layers) markedly absorbed a broad range of UV light. This result indicates that the cyst wall evidently has UV-cut function. These results support that the cyst wall and NSPs of C. cucullus play a role in the shielding of the cell components from UV light.
Corliss J. O., Esser S. C. (1974) Comments on the role of the cyst in the life cycle and survival of free-living protozoa. Trans. Amer. Micros. Soc. 93: 578–593
Foissner W., Stoeck T., Agatha S., Dunthorn M. (2011) Intraclass evolution and classification of the Colpodea (Ciliophora). J. Eukaryot. Microbiol. 58: 397–415
Funadani R., Sogame Y., Kojima K., Takeshita T., Yamamoto K., Tsujizono T., Suizu F., Miyata S., Yagyu K., Suzuki T., Arikawa M., Matsuoka T. (2016) Morphogenetic and molecular analyses of cyst wall components in the ciliated protozoan Colpoda cucullus Nag-1. FEMS Microbiol. Lett. 363: fnw203
Funatani R., Kida A., Watoh T., Matsuoka T. (2010) Morphological events during resting cyst formation (encystment) in the ciliated protozoan Colpoda cucullus. Protistology 6: 204–217
Lonnen J., Putt K. S., Kernick E. R., Lakkis C., May L., Pugh R. B. (2014) The efficacy of Acanthamoeba cyst kill and effects upon contact lenses of a novel ultraviolet lens disinfection system. Am. J. Ophthalmol. 158: 460–468
Maeda H., Akematsu T., Fukui R., Matsuoka T. (2005) Studies on the resting cyst of ciliated protozoan Colpoda cucullus: resistance to temperature and additional inducing factors for en-or excystment. J. Protozool. Res. 15: 7–13
Matsuoka K., Funadani R., Matsuoka T. (2017) Tolerance of Colpoda cucullus resting cysts to ultraviolet irradiation. J. Protozool. Res. 27: 1–7
Müller H., Achilles-Day U. E. M., Day J. G. (2010) Tolerance of the resting cysts of Colpoda inflata (Ciliophora, Colpodea) and Meseres corlissi (Ciliophora, Spirotrichea) to desiccation and freezing. Eur. J. Protistol. 46: 133–142
Nicholson W. L., Galeano B. (2003) UV resistance of Bacillus anthracis spores revisited: Validation of Bacillus subtilis spores as UV surrogates for spores of B. anthracis Sterne. Appl. Environ. Microb. 69: 1327–1330
Sogame Y., Kida A., Matsuoka T. (2011) Possible involvement of endocyst in tolerance of the resting cyst of Colpoda cucullus against HCl. Afr. J. Microbiol. Res. 5: 4316–4320
Sogame Y., Kojima K., Takeshita T., Kinoshita E., Matsuoka T. (2014) Identification of differentially expressed water-insoluble proteins in the encystment process of Colpoda cucullus by twodimensional electrophoresis and LC-MS/MS analysis. J. Eukaryot. Microbiol. 61: 51–60
Sogame Y., Saito R., Koizumi R., Shimizu T., Ono T. (2019) Evidence of stress recovery in free-living ciliate Colpoda cucullus: The repair capability of resting cysts to damage caused by gamma irradiation. Acta Protozool. 58: 25–29
Taylor C. V., Strickland A. G. R. (1936) Effects of high vacua and extreme temperatures on the cysts of Colpoda cucullus. Physiol. Zool. 9: 15–26
Information: Acta Protozoologica, 2020, Volume 59, Issue 1, pp. 55 - 60
Article type: Original article
Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
Oncology Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Takamatsu 761-0793, Japan
Kochi Rehabilitation Institute, Tosa, Kochi, Japan
Department of Biological Science, Faculty of Science, Kochi University, Kochi, Japan
Published at: 2020
Received at: 18.12.2019
Accepted at: 09.03.2020
Article status: Open
Licence: CC BY-NC-ND
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