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Acta Protozoologica

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Novel Media for Lipid Production of Chlorococcum oleofaciens : A RSM Approach

Publication date: 25.07.2019

Acta Protozoologica, 2019, Volume 58, Issue 1, pp. 31 - 41

https://doi.org/10.4467/16890027AP.19.003.10834

Authors

,
J. Mercy Nisha Pauline
Department of Industrial Biotechnology, Government College Of Technology, Coimbatore-641013, Tamil Nadu, India
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Anant Achary
Department of Biotechnology, Kamaraj College of Engineering & Technology, Virudhunagar-626001, Tamil Nadu, India
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Abstract

The algal medium was optimized to increase the biomass and lipid production of Chlorococcum oleofaciens. The significant variables were screened and chosen from previously reported algal culture media using Plackett Burman Design (PBD). Optimization of the significant variables were performed using central composite  design. The Pareto chart for PBD revealed that the salts such as sodium bicarbonate, sodium nitrate, potassium nitrate and ferrous sulphate had enhanced the biomass and lipid production. The variables and its effect on the responses were further studied by central composite design (CCD). A new medium was formulated based  on the response surface methodology. The predicted concentration of NaHCO3, NaNO3, KNO3, MgSO4.7H2O were found to be 6.75 g/L, 0.75 g/L, 1.88 g/L and 0.35 g/L respectively. The actual and the predicted total lipid yield for the optimized media was around 0.74 g/L and 0.78 g/L respectively. The optimal medium has been named as AM medium. Growth and the lipid yields of C. oleofaciens were found higher in AM medium. The specific growth rates of C. oleofaciens in AM and CFTRI media were found to be 0.14 day-1 and 0.19 day-1 respectively. The biomass produced by the optimized AM medium was found to be 2.7 times greater compared to  the CFTRI medium. The lipid was extracted and GC-MS was performed which revealed that the fatty acids were predominantly of the class C15:0, C18:0, C16:0 and C12:0. It is concluded that besides lipid content, AM medium increased the cell number leading to the increase in biomass.

Received on 10th April, 2019; revised on 7th June, 2019; accepted on 20th June, 2019

References

Allen M. M., Stanier R. Y. (1968) Growth and division of some unicellular blue-green algae. J. Gen. Microbiol. 51: 199–202

Aizawa K., Miyachi S. (1986) Carbonic anhydrase and CO2 concentrating mechanisms in microalgae and cyanobacteria. FEMS Microbiology Reviews. 2: 215–233

Andrés F. Barajas-Solano, Guzmán-Monsalve A., Viatcheslav Kafarov (2016) Effect of Carbon–Nitrogen Ratio for the Biomass Production, Hydrocarbons and Lipids on Botryoccus braunii UIS 003. Chem. Engg. Transactions. 49: 247–252

Bernard J., Finkle, Appleman D. (1952) The effect of magnesium concentration on growth of Chlorella. Plant Physiol. 28: 664–673

Bischoff H. W., Bold H. C. (1963) Phycological Studies IV: Some soil algae from Enchanted Rock and related algal species. Univ Texas Publ. pp. 6318: 1–95

Cheng Y., Lu Y., Gao C., Wu Q. (2009) Alga-based biodiesel production and optimization using sugar cane as the feedstock. Energy  Fuels 23: 4166–4173

Cho H. U., Kim Y. M., Choi Y. N., Xu X., Shin D. Y., Park J. M. (2015) Effects of pH control and concentration on microbial oil production from Chlorella vulgaris cultivated in the effluent of a low-cost organic waste fermentation system producing volatile fatty acids. Bioresour. Technol. 184: 245–250

Franklin S.E., Mayfield S.P. (2004) Prospects for molecular farming in the green alga Chlamydomonas reinhardtii. Curr. Opin. Plant Bio. 7:150–165

Ge S., Champagne P., Plaxton W. C., Leite G. B., Marazzi F. (2017) Microalgal cultivation with waste streams and metabolic constraints to triacylglycerides accumulation for biofuel production. Biofuels, Bioproducts and Biorefining 11: 325–343

Ghirardi M. L., Zhang L., Lee J. W., Flynn T., Seibert M., Greenbaum E., Melis A. (2000) Microalgae: a green source of renewable hydrogen. Trends Biotechnol. 18: 506–511

Golueke C. G., Oswald W. J., Gotaas H. B. (1957) Anaerobic digestion of algae. Appl. Microbiol. 5: 47–55

Guillard R. R., Ryther J. H. (1962) Studies on marine planktonic diatoms I. Cyclotella nana Hustedt and Detonula confervacea (Cleve). Gran. Can. J. of Microbiol. 8: 229–239

Gutierrez L. F., Ratti C., Belkacemi K. (2008) Effects of drying method on the extraction yields and quality of oils from Quebec Sea buckthorn (Hippophae rhamnoides L.) seeds and pulp. Food Chem. 106: 896–904

Hare P. D., Cress W. A., Van-staden J. (1998) Dissecting the roles of osmolyte accumulation during stress. Plant Cell. Environ. 21: 535–554

Hasegawa P. M., Bressan R. A., Zhu J. K., Bohnert H. J. (2000) Plant cellular and molecular response to high salinity. Physiol Plant Mol. Biol. 51: 463–499

Hoque M. A., Okuma E., Banu M. N. A., Nakamura Y., Shimoishi Y., Murata Y. (2007) Exogenous proline mitigates the detrimental effects of salt stress more than the betaine by increasing antioxidant enzyme activities. J. Plant Physiol. 164: 553–561

Hu Q., Sommerfeld M., Jarvis E., Ghirardi M., Posewitz M., Seibert M., Darzins A. (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. The Plant J. 54: 621–639

Hu Q., Sommerfeld M., Jarvis E., Ghirardi M., Posewitz M., Seibert M., Darzins A. Illman M., Scragg A. H., Shales S. W. (2000) Increase in Chlorella Strains Calorific Values when Grown in Low Nitrogen Medium. Enz. and Microbial. Technol. 27: 631–635

Imran P., Kaumeel C., Tonmoy G., Chetan P., Rahulkumar M., Sandhya M. (2015) Bicarbonate supplementation enhanced biofuel production potential as well as nutritional stress mitigation in the microalgae Scenedesmus sp. CCNM 1077. Bioresour. Technol. 193: 315–323

Jeng Chen J., Li Y., Lai W. (2014) Application of experimental design methodology for optimization of biofuel production from microalgae. Biomass and Bioenergy 64: 11–19.

Jingya L., Changhao L., Christopher Q. Lan, Dankui L. (2018) Effects of sodium bicarbonate on cell growth, lipid accumulation, and morphology of Chlorella vulgaris. Microb. Cell Fact. 17: 111–121

Kandhro A., Sherazi S. T. H., Mahesar S. A., Bhanger M. I., Younis T. M., Rauf A. (2008) GC-MS quantification of fatty acid profile including trans FA in the locally manufactured margarines of Pakistan. Food Chem.109: 207–211

Kong W., Song H., Cao Y., Yang H., Hua S., Xia C. (2011) The characteristic of biomass production, lipid accumulation and chlorophyll biosynthesis of Chlorella vulgaris under mixotrophic cultivation. Afr. J. Biotechnol. 10: 11620–11630

Kuan Chen C., Ming R., Kimberly L. Ogden (2013) Statistical optimization of culture media for growth and lipid production of Chlorella protothecoides UTEX 250. Bioresor. Technol 128: 44–48

Liu Z. L., Saha B. C., Slininger P. J. (2008) Lignocellulosic biomass conversion to ethanol by Saccharomyces In: Bioenergy, ASM Press, Washington, DC. 17–36

Liu Huang J., Sun Z. (2011) Differential lipid and fatty acid profiles of photoautotrophic and heterotrophic Chlorella zofingiensis: assessment of algal oils for biodiesel production. Bioresour. Technol. 102: 106–110

Luveshan R., Abhishek G., Ismail R., Faizal B (2014) The optimization of biomass and lipid yields of Chlorella sorokiniana when using wastewater supplemented with different nitrogen sources. Bioresource Technol. 168: 127–135

Marchetti J. M., Miguel V. U., Errazu A. F. (2007) Possible Methods For Biodiesel Production. Renew. Sus. Energy Rev. 11: 1300–1311

Martinez M. R., Chakroff R. P., Pantastico J. B. (1975) Direct phytoplankton counting techniques. Philippine agriculturist. 59: 43–50

Olmstead I. L., Hill D. R., Dias D. A., Jayasinghe N. S., Callahan D. L., Kentish S. E., Martin G. J. (2013) A quantitative analysis of microalgal lipids for optimization of biodiesel and omega-3 production. Biotechnol. Bioeng. 110: 2096–2104

Pablo C. Giordano, Alejandro J. Beccaria, Héctor C. Goicoechea. (2014) Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor. Bioresour. Technol. 158: 269–277

Piorreck M., Baasch K.H., Pohl P. (1984) Biomass Production, Total Protein, Chlorophylls, Lipids and Fatty Acids of Fresh Water Green and Blue-Green Algae under Different Nitrogen Regimes. Phytochem. 23: 207–216

Plackett R.L., Burman J.P. (1946) The design of optimum multifactorial experiments. Biometrika 33: 305–325

Ramírez López C., Chairez I., Fernández Linares L. (2016) A novel culture medium designed for the simultaneous enhancement of biomass and lipid production by Chlorella vulgaris UTEX 26. Bioresour. Technol. 212: 207–216

Rodriguez A. (2011) Enhancement of Lutein Production in Chlorella sorokiniana (chorophyta) by improvement of culture conditions and random mutagenesis. Mar. Drugs 9: 1607–1624

Rekha S., Gajendra P. Singh, Vijendra K. Sharma (2011) Comparison of Different Media Formulations on Growth, Morphology and Chlorophyll Content of Green Alga, Chlorella Vulgaris. Intl. J. of Pharma and Bio Sciences. 2: 506–516

Sivaramakrishnan R., Incharoensakdi A. (2018) Utilization of microalgae feedstock for concomitant production of bioethanol and biodiesel. Fuel 217: 458–466

Sivaramakrishnan R., Incharoensakdi A. (2017) Production of methyl ester from two microalgae by two-step transesterification and direct transesterification. Environ. Sci. Pollut. Res. 24: 4950–4963

Shay E.G. (1993) Diesel fuel from vegetable oils: Status and Opportunities. Biomass Bioenergy 4: 227–242

Stein J (ED.) (1973) Handbook of Phycological methods. Culture methods and growth measurements. Cambridge University Press. 448 pp.

Takagi M., Watanabe K., Yamaberi K., Yoshida Y. (2000) Limited feeding of potassium nitrate for intracellular lipid and triglyceride accumulation of Nannochloris sp. UTEX LB1999. Appl. Microbiol. Biotechnol. 54: 112–117

Tiwari K., Kumar A., Raheman H. (2007) Biodiesel production from Jatropha curcas with high free fatty acids: an optimized process. Biomass Bioenergy 31: 569–575

Tornabene T. G., Holzer G., Lien S., Burris N. (1983) Lipid Composition Of The Nitrogen Starved Green Alga Neochloris oleoabundans. Enzyme Microb. Technol. 5: 435–440

Turcotte, G., Kosaric, N. (1989) The effect of C/N ratio on lipid production by Rhodosporidium toruloides ATCC 10788. Biotechnol. Lett. 9: 637–642

Van der Laaka W. W. M., Raven R. P. J. M., Verbong G. P. J. (2007) Strategic Niche Management for Biofuels: Analysing Past Experiments for Developing New Biofuel Policies. Energy Policy 35: 3213–3225

Venkatraman L. V., Becker E. W. (1985) Biotechnology and utilization of algae – The Indian experience. Central Food Technological Research Institute, Mysore, India.

WalneP.R. (1970) Studies on the food value of nineteen genera of algae to juvenile bivalves of the genera Ostrea, Crassostrea, Mercenaria, and Mytilis. Fish. Invest. 26: 162

Wu Q., Miao X. (2006) Biodiesel production from heterotrophic microalgal oil. Bioresour. Technol. 97: 841–846

Xin L., Hong-ying H., Ke G., Ying-xue S. (2010) Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp. Bioresour. Technol. 101: 5494–5500

Xiong W., Chunfang G., Dong Y., Chao W., Qingyu W. (2010) Double CO2 fixation in photosynthesis–fermentation model enhances algal lipid synthesis for biodiesel production. Bioresour. Technol. 101: 2287–2293

Yang F., Long L., Sun X., Wu H., Li T., Xiang W. (2014) Optimization of Medium Using Response Surface Methodology for Lipid Production by Scenedesmus sp. Mar. Drugs. 12: 1245–1257

Zarrouk C. (1966) Contribution a l’étude du cyanophycée. Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthèse de spirulina maxima (setch et gardner) geitl. paris: Faculte des Sciences, Universite de Paris.

Information

Information: Acta Protozoologica, 2019, pp. 31 - 41

Article type: Original research article

Authors

Department of Industrial Biotechnology, Government College Of Technology, Coimbatore-641013, Tamil Nadu, India

Department of Biotechnology, Kamaraj College of Engineering & Technology, Virudhunagar-626001, Tamil Nadu, India

Published at: 25.07.2019

Article status: Open

Licence: CC BY-NC-ND  licence icon

Percentage share of authors:

J. Mercy Nisha Pauline (Author) - 50%
Anant Achary (Author) - 50%

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