FAQ
Jagiellonian University logo

Morphological and Molecular Identification of Isospora sepetibensis (Chromista: Miozoa: Eimeriidae) from a New Host, Trichothraupis melanops (Passeriformes: Thraupidae: Tachyphoninae) in South America

Publication date: 25.07.2019

Acta Protozoologica, 2019, Volume 58, Issue 1, pp. 17 - 23

https://doi.org/10.4467/16890027AP.19.007.10838

Authors

,
Jhon Lennon Genovez-Oliveira
Programa de Pós-Graduação em Biologia Animal, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Brazil
All publications →
,
Sergian V. Cardozo
Programa de Pós-Graduação em Biomedicina Translacional, Universidade do Grande Rio, Rua Professor José de Souza Herdy 1160, 25071-202 Duque de Caxias, RJ, Brazil
All publications →
,
Águida A. De Oliveira
Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, UFRRJ, RJ, Brazil
All publications →
,
Viviane M. De Lima
Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, UFRRJ, RJ, Brazil
All publications →
,
Ildemar Ferreira
Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, UFRRJ, RJ, Brazil
All publications →
Bruno P. Berto
Departamento de Parasitologia Animal, Instituto de Veterinária, Seropédica, RJ, Brasil – CNPq fellowship
All publications →

Titles

Morphological and Molecular Identification of Isospora sepetibensis (Chromista: Miozoa: Eimeriidae) from a New Host, Trichothraupis melanops (Passeriformes: Thraupidae: Tachyphoninae) in South America

Abstract

Isospora sepetibensis Berto, Flausino, Luz, Ferreira and Lopes, 2008 is a protozoan coccidian parasite (Chromista: Miozoa: Coccidiomorphea: Coccidia) that was originally described from Brazilian tanagers Ramphocelus bresilius (Linnaeus, 1766) in the Marambaia Island in the Coast of the State of Rio de Janeiro. In the current  work, this species was identified from black-goggled tanagers Trichothraupis melanops (Vieillot, 1818) in the Itatiaia National Park, which is a protected area with a high degree of vulnerability in the interior of the State of Rio de Janeiro, distant in more than 100 km of the type-locality. Its oocysts are sub-spherical to elongate  ovoidal, 25.9 × 20.7 μm with smooth, bi-layered wall, ~ 1.3 μm and length/width ratio of 1.1–1.4 (1.26). Micropyle and oocyst residuum absent, but one or two polar granules are present. Sporocysts are ellipsoidal, 16.8 × 10.3 μm, with both Stieda and sub-Stieda bodies. Sporocyst residuum present  and sporozoites with refractile body and nucleus. Molecular analysis was conducted at the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. This new isolate exhibited similarity greater than 98% with Isospora spp. isolates from other Neotropical passerines and with an Isospora sp. pseudoparasite of voles of Eurasia. This is the first coccidian  parasite from a New World tanager to have a molecular identification of the cox1 gene.

Article data

 
Received on 28th March, 2019; revised on 8th July, 2019; accepted on 8th July, 2019

References

Download references

Adkesson M. J., Zdziarski M., Little S. E. (2005) Atoxoplasmosis in tanagers. J. Zoo Wildl. Med., 36: 265–272

Bensch S., Stjernman M., Hasselquist D., Örjan Ö., Hannson B., Westerdahl H., Pinheiro R. T. (2000) Host specificity in avian blood parasites: a study of Plasmodium and Haemoproteus mitochondrial DNA amplified from birds. Proc. R. Soc. Lond. B Biol. Sci., 267: 1583–1589

Berto B. P., Flausino W., Luz H. R., Ferreira I., Lopes C. W. G. (2008) Three new coccidian parasites of Brazilian tanager (Ramphocelus bresilius dorsalis) from South America. Acta Protozool., 47: 77–81

Berto B. P., Flausino W., McIntosh D., Teixeira-Filho W. L., Lopes C. W. G. (2011a) Coccidia of New World passerine birds (Aves: Passeriformes): a review of Eimeria Schneider, 1875 and Isospora Schneider, 1881 (Apicomplexa: Eimeriidae). Syst. Parasitol., 80: 159–204

Berto B. P., Luz H., Flausino W., Teixeira-Filho W. L., Ferreira I., Lopes C. W. G. (2011b) Isosporoid Coccidia (Apicomplexa: Eimeriidae) parasites of tanagers (Passeriformes: Thraupidae) from the Marambaia Island, Brazil. Pesq Vet Bras., 31: 798–805

Berto B. P., Lopes C. W. G. (2013) Distribution and dispersion of coccidia in wild passerines of the Americas. In: Ruiz L., Iglesias L. (Eds), Birds: evolution and behavior, breeding strategies, migration and spread of disease. Nova Science Publishers, New York. pp. 47–66

Berto B. P., McIntosh D., Lopes C. W. G. (2014) Studies on coccidian oocysts (Apicomplexa: Eucoccidiorida). Rev. Bras. Parasitol. Vet., 23: 1–15

Dolnik O. V., Palinauskas V., Bensch S. (2009) Individual oocysts of Isospora (Apicomplexa: Coccidia) parasites from avian feces: from photo to sequence. J. Parasitol., 95: 169–174

Dolnik O. V., Dolnik V. R., Bairlein F. (2010) The effect of host foraging ecology on the prevalence and intensity of coccidian infection in wild passerine birds. Ardea, 98: 97–104

Duszynski D., Wilber P. (1997) A guideline for the preparation of species descriptions in the Eimeridae. J Parasitol., 83: 333–336

Fayer R. (1980) Epidemiology of protozooan infection: the Coccidia. Vet Parasitol., 6: 75–103

Gardner S., Duszynski D. (1990) Polymorphism of eimerian oocysts can be a problem in naturally infected hosts: an example from subterranean rodents in Bolivia. J Parasitol., 76: 805–811

Hafeez M. A., Stasiak I., Delnatte P., El-Sherry S., Smith D. A., Barta J. R. (2014) Description of two new Isospora species causing visceral coccidiosis in captive superb glossy starlings, Lamprotornis superbus (Aves: Sturnidae). Parasitol. Res., 113: 3287–3297

ICMBIO (2019) Parque Nacional do Itatiaia. Available from: . Accessed 02 Mar 2019

Illera J. C., Fernández‐Álvarez Á., Hernández‐Flores C. N., Foronda P. (2015) Unforeseen biogeographical patterns in a multiple parasite system in Macaronesia. J. Biogeogr., 42: 1858–1870

Isler M. L., Isler P. R. (1987) The Tanagers: Natural History, Distribution, and Identification. Smithsonian Institution Press, Washington

Ogedengbe J. D., Hanner R. H., Barta J. R. (2011) DNA barcoding identifies Eimeria species and contributes to the phylogenetics of coccidian parasites (Eimeriorina, Apicomplexa, Alveolata). Int. J. Parasitol., 41: 843–850

Perkins S. L., Shall J. J. (2002) A molecular phylogeny of malarial parasites recovered from cytochrome b gene sequences. J. Parasitol., 88: 972–978

Ridgely R. S., Tudor G. (1989) The birds of South America: the oscines passerines. University of Texas, Austin

Rodrigues M. B., Oliveira J. L. G., Silva-Carvalho L. M., Pastura D. G. N., Gomes J. V., Oliveira M. S., Siqueira P. B., Oliveira A. A., Lima V. M., Ferreira I., Berto B. P. (2019). The vulnerable Sporophila frontalis (Verreaux) and Haplospiza unicolor Cabanis as new hosts for Isospora sporophilae Carvalho-Filho, Meireles, Ribeiro & Lopes, 2005 (Eimeriidae) in Brazil. Syst. Parasitol. [in press]

Ruggiero M. A., Gordon D. P., Orrell T. M., Bailly N., Bourgoin T., Brusca R. C., Cavalier-Smith T., Guiry M. D., Kirk P. M. (2015) Correction: A Higher Level Classification of All Living Organisms. PLoS One 10: e0130114

Schrenzel M. D., Maalouf G. A., Gaffney P. M., Tokarz D., Keener L. L., Mcclure D., Griffey S., Mcaloose D., Rideout B. A. (2005) Molecular characterization of isosporoid Coccidia (Isospora and Atoxoplasma spp.) in passerine birds. J. Parasitol., 91: 635–647

Sick H. (1997) Ornitologia Brasileira. Nova Fronteira, Rio de Janeiro Silva-Carvalho L. M., Pastura D. G. N., Gomes J. V., Siqueira P. B., Rodrigues M. B., Lima V. M., Berto B. P. (2018a). Isospora lopesi n. sp. (Protozoa: Apicomplexa: Eimeriidae) from the eastern white-throated spadebill Platyrinchus mystaceus Vieillot (Passeriformes: Tyranni: Tyrannidae) in South America. Syst. Parasitol., 95: 455–463

Silva-Carvalho L. M., Pastura D. G. N., Rodrigues M. B., Gomes J. V., Oliveira M. S., Siqueira P. B., Oliveira J. L. G., Soares S. S., Oliveira A. A., Lima V. M., Ferreira I., Berto B. P. (2018b). Isospora sagittulae McQuistion & Capparella (1992 (Apicomplexa: Eimeriidae) from antbirds (Passeriformes: Thamnophilidae) in the Amazon and Atlantic Forest of Brazil: with notes on its distribution and dispersion in the Neotropical region. Parasitol. Res., 117: 2635–2641

Tamura K., Dudley J., Nei M., Kumar S. (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24: 1596–1599

Trefancová A., Mácová A., Kvičerová J. (2019) Isosporan Oocysts  in the Faeces of Bank Voles (Myodes glareolus; Arvicolinae, Rodentia): Real Parasites, or Pseudoparasites?. Protist, 170: 104–120

Waldenström J., Bensch S., Kiboi S., Hasselquist D., Ottosson U. (2002) Cross‐species infection of blood parasites between resident and migratory songbirds in Africa. Mol. Ecol., 11: 1545–1554

Williams R. B., Thebo P., Marshall R. N., Marshall J.A. (2010) Coccidian oöcysts as type-specimens: long-term storage in aqueous potassium dichromate solution preserves DNA. Syst. Parasitol., 76: 69–76

Yang R., Brice B., Elliot A., Ryan U. (2015) Isospora serinuse n. sp. (Apicomplexa: Eimeriidae) from a domestic canary (Serinus canaria forma domestica) (Passeriformes: Fringillidae) in Western Australia. Exp. Parasitol., 159: 59–66

Yang R., Brice B., Jian F., Ryan U. (2016) Morphological and molecular characterization of Isospora manorinae n. sp. in a yellow-throated miner (Manorina flavigula wayensis) (Gould, 1840). Exp. Parasitol., 163: 16–23

Information

Information: Acta Protozoologica, 2019, Volume 58, Issue 1, pp. 17 - 23

Article type: Original article

Authors

Programa de Pós-Graduação em Biologia Animal, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Brazil

Programa de Pós-Graduação em Biomedicina Translacional, Universidade do Grande Rio, Rua Professor José de Souza Herdy 1160, 25071-202 Duque de Caxias, RJ, Brazil

Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, UFRRJ, RJ, Brazil

Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, UFRRJ, RJ, Brazil

Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, UFRRJ, RJ, Brazil

Departamento de Parasitologia Animal, Instituto de Veterinária, Seropédica, RJ, Brasil – CNPq fellowship

Published at: 25.07.2019

Article status: Open

Licence: CC BY-NC-ND  licence icon

Percentage share of authors:

Jhon Lennon Genovez-Oliveira (Author) - 16%
Sergian V. Cardozo (Author) - 16%
Águida A. De Oliveira (Author) - 16%
Viviane M. De Lima (Author) - 16%
Ildemar Ferreira (Author) - 16%
Bruno P. Berto (Author) - 20%

Article corrections:

-

Publication languages:

English