FAQ
Jagiellonian University logo

Evidence of Stress Recovery in Free-Living Ciliate Colpoda cucullus: The Repair Capability of Resting Cysts to Damage Caused by Gamma Irradiation

Publication date: 25.07.2019

Acta Protozoologica, 2019, Volume 58, Issue 1, pp. 25 - 29

https://doi.org/10.4467/16890027AP.19.006.10837

Authors

,
Yoichiro Sogame
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
All publications →
,
Ryota Saito
Department of Chemistry and Biotechnology, Kochi University, Kochi 780-8520, Japan
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
All publications →
,
Ryota Koizumi
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
All publications →
,
Taiga Shimizu
Department of Chemistry and Biotechnology, Kochi University, Kochi 780-8520, Japan
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
All publications →
Taiki Ono
National Institute of Technology Fukushima College, Iwaki, Fukushima Japan
All publications →

Titles

Evidence of Stress Recovery in Free-Living Ciliate Colpoda cucullus: The Repair Capability of Resting Cysts to Damage Caused by Gamma Irradiation

Abstract

In this study, we report that the unicellular free-living protist Colpoda cucullus in the resting cyst (cryptobiosis) repairs stress damage. We previously demonstrated that resting cysts of Colpoda cucullus have extreme tolerance to gamma irradiation and can revert to vegetative cells after irradiation. Such irradiated cysts gradually  excyst, suggesting that stress repair mechanisms are active during excystment or in the resting cyst. Herein we provide bioassay evidence that the rate of excystment of irradiated cysts is elevated by subsequent incubation, thereby indicating that cells injured by gamma irradiation can repair themselves in the resting cyst,  whereas irradiated dry cysts cannot.

Article data

Received on 8th April, 2019; revised on 28th June, 2019; accepted on 9th July, 2019

References

Download references

Azam E. I., Jay-Gerin J. P., Pain D. (2012) Ionizing radiationinduced metabolic oxidative stress and prolonged cell injury. Cancer Lett. 327: 48–60

Campbell C., Romero D. P. (1998) Identification and characterization of the RAD51 gene from the ciliate Tetrahymena thermophila. Nucleic Acids Res. 26: 3165–3172

Clegg S. J. (2001) Cryptobiosis: a peculiar state of biological organization. Comp. Biochem. and Physiol. B 128: 613–624

Close D. M., Nelson W. H., Bernhard W. A. (2013) DNA Damage by the Direct Effect of Ionizing Radiation: Products Produced by Two Sequential One-Electron Oxidations. J. phys. chem. 117: 12608–12615

DiRuggiero J., Santangelo N., Nackerdien Z., Ravel J., Robb F. T. (1997) Repair of extensive ionizing-radiation DNA damage at 95 degrees C in the hyperthermophilic archaeon Pyrococcus furiosus. J. Bacteriol. 179: 4643–4645

Funatani R., Kida A., Watoh T., Matsuoka T. (2010) Morphological events during resting cyst formation in the ciliate Colpoda cucullus. Protistlogy 6: 204–217

Gutiérrez J. C., Martín-González A. Matsusaka T. (1990) Towards a generalized model of encystment (cryptobiosis) in ciliates: a review and a hypothesis. BioSystems. 24: 17–24

Gutiérrez J. C., Callejas S., Borniquel S., Benítez L., Martín- González A. (2001) Ciliate cryptobiosis: a microbial strategy against environmental starvation. Int. Microbiol. 4: 151–157

Gutiérrez J. C., Díaz S., Ortega R., Martín-González A. (2003) Ciliate resting cyst walls: A comparative review. Recent Res. Devel. Microbiol. 7: 361–379

Hallwell B., Gutteridge J. (1999) Free Radicals in biology and medicine. Oxford University Press, Oxford

Imlay J. A., Linn S. (1988) DNA damage and oxygen radical toxicity. Science 240: 4857

Jung K., Lim S., Bahn Y. (2017) Microbial radiation-resistance mechanisms. J. Microbiol. 55: 499–507

Keilin D. (1959) The problem of anabiosis or latent life: history and current concept. Proc. Roy. Soc. Lond. B 150: 149–191

Richter J. R., Kasten B. B., Zinn K. R. (2016) Imaging and Adenoviral gene therapy. In Curiel DT (ed) Adenovital vectors for gene therapy. Elsevier, pp. 767–802

Roca A. I., Cox M. M. (1997) RecA protein: structure, function, and role in recombinational DNA repair. Prog. Nucleic Acid Res. Mol. Biol. 56: 129–222

Ryter S. W., Kim H. P., Hoetzel A., Park J. W., Nakahira K., Wang X., Choi A. M. Mechanisms of Cell Death in Oxidative Stress. Antioxid. Redox. Signal. 9: 49–89

Sogame Y., Kida A., Matsuoka T. (2011) Possible involvement of endocyst in tolerance of the resting cyst of Colpoda cucullus against HCl. Afr. J. Microbiol. Res. 5: 4316–4320

Sogame Y., Kojima K., Takeshita T., Kinoshita E., Funadani R., Matsuoka T. (2013) Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus. Microbes Environ. 28: 388–390

Sogame Y., Kojima K., Takeshita T., Kinoshita E., Matsuoka T. (2014) Identification of differentially expressed water-insoluble proteins in the encystment process of Colpoda cucullus by two-dimensional electrophoresis and LC-MS/MS analysis. J. Eukaryot. Microbiol. 61: 51–60

Slaveykova V., Sonntag B., Gutiérrez J. C. (2016) Stress and Protists: No life without stress. Eur. J. Protistol. 55: 39–49

Taylor C. V., Strickland A. G. R. (1936) Effects of high vacua and extreme temperatures on cysts of Colpoda cucullus. Physiol. Zool. 9: 15–26

Uspenskaya Z. I., Lozia-Lozinsky L. K. (1979) Antigen rearrangements in Colpoda maupasi cells after freezing at −196°C, and after shortwave ultraviolet irradiation. Cryobiology 16: 542–549

Verni F., Rosati R. (2011) Resting cysts: a survival strategy in protozoa Ciliophora. Ital. J. Zool.78: 134–145

Information

Information: Acta Protozoologica, 2019, Volume 58, Issue 1, pp. 25 - 29

Article type: Original article

Authors

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

Department of Chemistry and Biotechnology, Kochi University, Kochi 780-8520, Japan

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

Department of Chemistry and Biotechnology, Kochi University, Kochi 780-8520, Japan

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

National Institute of Technology Fukushima College, Iwaki, Fukushima Japan

Published at: 25.07.2019

Article status: Open

Licence: CC BY-NC-ND  licence icon

Percentage share of authors:

Yoichiro Sogame (Author) - 20%
Ryota Saito (Author) - 20%
Ryota Koizumi (Author) - 20%
Taiga Shimizu (Author) - 20%
Taiki Ono (Author) - 20%

Article corrections:

-

Publication languages:

English