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Certesella larai (Amoebozoa: Arcellinida: Hyalospheniformes) a new soil testate amoeba species from the Dominican Republic and Chile challenges the definition of genera Certesella and Porosia

Publication date: 14.02.2022

Acta Protozoologica, 2021, Volume 60, pp. 61 - 75

https://doi.org/10.4467/16890027AP.21.007.15381

Authors

,
Anatoly Bobrov
Department of Soil Science, Lomonosov Moscow State University, Leninskiye gory, Moscow, Russia, 119991
https://orcid.org/0000-0003-2240-384X Orcid
All publications →
,
Clément Duckert
Laboratory of Soil Biodiversity, University of Neuchâtel, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland
https://orcid.org/0000-0001-9386-2950 Orcid
All publications →
Edward A. D. Mitchell
Laboratory of Soil Biodiversity, University of Neuchâtel, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland
https://orcid.org/0000-0003-0358-506X Orcid
All publications →

Abstract

Microbial diversity is known to be huge but remains only partly documented. Testate amoebae are a relatively well studied group or free-living protists that build morphologically characteristic shells on which the taxonomy of this group is mainly based. Among testate amoebae, the Hyalospheniformes are especially well documented and are a model group for microbial biogeography. Here we describe a new species of genus Certesella from Sphagnum mosses and forest litter samples collected in the Dominican Republic and Chile. We name this species Certesella larai to honour our esteemed colleague Enrique Lara. This species bears two large pores connected by tube, which are characteristic of the genera Porosia and Certesella. The new species fits best in the latter genus given the presence of a distinct, sometimes denticulated, neck with a bulge that is absent in genus Porosia but occurs in Certesella. Its elongated shape (length/breadth ratio 2.03–2.58) only overlaps with C. certesi, which is distinguishable by the presence of a distinct longitudinal groove in the neck region that is absent from the new species. Interestingly most specimens lacked the internal teeth which are usually present in members of genus Certesella. As such, while the morphology of C. larai strongly suggests a belonging to genus Certesella, molecular analyses are still required to confirm its phylogenetic position, clarify the relationships among all species in genera Porosia and Certesella and the definition and validity of these two generaThe presence of this new species in two rather distant regions – Caribbean and central Chile – suggests it is likely widespread, but possibly rare. However, this perception may be due to a sampling bias in favour of Sphagnum mosses and wet and organic-rich habitats. This illustrates the need for a more systematic sampling effort to document the full and mostly unknown taxonomic diversity of soil protists.

AUTHORS CONTRIBUTIONS

EM collected samples in the Dominican Republic (together with Enrique Lara). AB, CD and EM performed LM observations, imaging and biometrical measurements. AB performed SEM analyses. EM prepared the tables and figures and wrote the paper with input from AB and CD.

ACKNOWLEDGEMENTS
We thank Dimaris Acosta Mercado for organising the joint sampling trip in the Dominican Republic funded by NSF DEB grant no. 0640052 and under permit no. 00805 Secretaría de Estado y Medio Ambiente y Recursos Naturales of the Dominican Republic. Enrique Lara kindly provided the sample from Chile and helped EM collect samples in the Dominican Republic. This research was supported by the Russian Foundation for Basic Research № 19-05-5009320. Further funding was provided by Moscow State.
University and the University of Neuchâtel. We thank two anonymous
reviewers who provided useful comments on the manuscript.

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Information

Information: Acta Protozoologica, 2021, pp. 61 - 75

Article type: Original research article

Authors

https://orcid.org/0000-0003-2240-384X

Anatoly Bobrov
Department of Soil Science, Lomonosov Moscow State University, Leninskiye gory, Moscow, Russia, 119991
https://orcid.org/0000-0003-2240-384X Orcid
All publications →

Department of Soil Science, Lomonosov Moscow State University, Leninskiye gory, Moscow, Russia, 119991

https://orcid.org/0000-0001-9386-2950

Clément Duckert
Laboratory of Soil Biodiversity, University of Neuchâtel, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland
https://orcid.org/0000-0001-9386-2950 Orcid
All publications →

Laboratory of Soil Biodiversity, University of Neuchâtel, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland

https://orcid.org/0000-0003-0358-506X

Edward A. D. Mitchell
Laboratory of Soil Biodiversity, University of Neuchâtel, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland
https://orcid.org/0000-0003-0358-506X Orcid
All publications →

Laboratory of Soil Biodiversity, University of Neuchâtel, Rue Emile-Argand 11, 2000 Neuchâtel, Switzerland

Published at: 14.02.2022

Received at: 09.04.2021

Accepted at: 14.09.2021

Article status: Open

Licence: CC BY  licence icon

Percentage share of authors:

Anatoly Bobrov (Author) - 33%
Clément Duckert (Author) - 33%
Edward A. D. Mitchell (Author) - 34%

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Publication languages:

English

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