Ultrastructure and Molecular Phylogeny of Mrazekia macrocyclopis sp. n. (Microsporidia, Mrazekiidae), a Microsporidian Parasite of Macrocyclops albidus (Jur.) (Crustacea, Copepoda)
cytuj
pobierz pliki
RIS BIB ENDNOTEChoose format
RIS BIB ENDNOTEUltrastructure and Molecular Phylogeny of Mrazekia macrocyclopis sp. n. (Microsporidia, Mrazekiidae), a Microsporidian Parasite of Macrocyclops albidus (Jur.) (Crustacea, Copepoda)
Publication date: 2010
Acta Protozoologica, 2010, Volume 49, Issue 1 , pp. 75 - 84
Authors
Ultrastructure and Molecular Phylogeny of Mrazekia macrocyclopis sp. n. (Microsporidia, Mrazekiidae), a Microsporidian Parasite of Macrocyclops albidus (Jur.) (Crustacea, Copepoda)
The ultrastructure and molecular phylogeny of a new microsporidium Mrazekia macrocyclopis sp.n., a parasite of the copepod Macrocyclops albidus (Jur.) in North-West of Russia are described. All stages of its life cycle are diplokaryotic. Fresh spores are rod-shaped and 7.3–10.5 × 1.6–2.3 μm in size. Spore ultrastructure is typical of Mrazekia. The polar tube consists of the anterior clavate manubrium followed by a thin filament arranged in 3.5–4.5 nearly vertical coils. Spores are enclosed in individual sporophorous vesicles. SSU rDNA sequence analysis showed attribution of the new species to a cluster of microsporidia infecting insects (Cystosporogenes, Endoreticulatus), microsrustaceans (Glugoides), vertebrates (Vittaforma) and ciliates (Euplotespora) nested within the clade IV sensu Vossbrinck, Debrunner- Vossbrinck (2005). Mrazekia macrocyclopis is not therefore closely related to Bacillidium vesiculoformis, another microsporidium with rod-shaped spores, and the polyphyletic nature of the family of Mrazekiidae is obvious.
Canning E. U., Vávra J. (2000) Phylum Microsporida Balbiani, 1882. In: An illustrated guide of the Protozoa, (Eds. J. J. Lee, G. F. Leedale, P. C. Bradbury). Society of Protozoologists, Lawrence, KS, 1: 39–126
Faye N., Toguebaye B. S., Bouix G. (1991) Microfilum lutjani n.g., n.sp. (Protozoa, Microsporida), a gill parasite of the Golden African Snapper Lutjanus Fulgens (Valenciennes, 1830) (Teleost Lutjanidae): developmental cycle and ultrastructure. J. Protozool. 38: 30–40
Hall T. A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. 41: 95–98
Kudo R. (1924) A biologic and taxonomic study of the Microsporidia. Illin. Biol. Monograph. 9: 1–268
Larsson R., Vávra J., Schrevel J. O. (1993) Bacillidium cyclopis Vávra, 1962 – Desription of the ultrastructural cytology and transfer to the genus Mrazekia Leger and Hesse, 1916 (Microspora, Mrazekiidae). Eur. J. Protistol. 29: 49–60
Leger L., Hesse E. (1916) Mrazekia, genre nouveau de Microsporidies a spores tubileuses. C. R. Soc. Biol. 79: 345–348Nilsen F. (1999) Small subunit rDNA phylogeny of Bacillidium sp. (Microspora, Mrazekidae) infecting oligochaets. Parasitol. 118: 553–558
Posada D. (2008) jModeltest: Phylogenetic Model Averaging. Mol. Biol. Evol. 25: 1253–1256
Ronquist F., Huelsenbeck J. P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 19: 1572–1574
Sambrook J., Fritsch E., Maniatis T. (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Swofford D. L. (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer Associates, Sunderland, MA
Vávra J. (1962) Bacillidium cyclopis n. sp. (Cnidospora, Microsporidia), a new parasite of copepods. Vestn. Česk. Spol. Zool. 26: 295–299
Vávra J. (1976) Structure of microsporidia. In: Comparative pathobiology I, (Eds. I. A. Bulla Jr., T. C. Cheng). Plenum Press, New York–London, 1–85
Vávra J., Larsson R. (1999) Structure of the Microsporidia. In: The Microsporidia and Microsporidiosis, (Eds. M. Wittner, L. M. Weiss). ASM Press, Washington, 7–84
Voronin V. N. (1986) The microsporidia of crustaceans. Protozoology (Leningrad). 10: 137–165 (in Russian)
Voronin V. N. (1990) The effect of methods of the host preparation on the Microsporidian extrasporal coat (Microsporidia). Parazitologya. 3: 242–244 (in Russian)
Voronin V. N. (1999) The microsporidia of freshwater invertebrates and fish of Russia. The thesis of doctoral dissertation, Saint-Petersburg, 45 pp. (in Russian)
Vossbrinck C. R., Debrunner-Vossbrinck B. A. (2005) Molecular phylogeny of the Microsporidia: ecological, ultrastructural and taxonomic considerations. Folia Parasitologica 52: 131–142
Weiss L. H., Vossbrinck C. R (1999) Molecular biology, molecular phylogeny, and molecular diagnostic approaches to the microsporidia. In: The Microsporidia and Microsporidiosis, (Eds. M. Wittner, L. M. Weiss). ASM Press, Washington, 129–171
Weiss L. M., Zhu X., Cali A., Tanowitz H. B., Wittner M. (1994) Utility of microsporidian rRNA in diagnosis and phylogeny: a review. Folia Parasitol. 41: 81–90
Information: Acta Protozoologica, 2010, Volume 49, Issue 1 , pp. 75 - 84
Article type: Original article
All-Russian Institute of Plant Protection, St. Petersburg, Pushkin, Russia
All-Russian Institute of Plant Protection, St. Petersburg, Pushkin, Russia
State Research Institute of Lake and River Fisheries, St. Petersburg, Russia;
I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia
All-Russian Institute of Plant Protection, St. Petersburg, Pushkin, Russia
All-Russian Institute of Plant Protection, St. Petersburg, Pushkin, Russia
Published at: 2010
Article status: Open
Licence: None
Percentage share of authors:
Article corrections:
-Publication languages:
EnglishView count: 1555
Number of downloads: 1331