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Spore dimorphism in Nosema pyrausta (Microsporidia, Nosematidae): from morphological evidence to molecular genetic verification

Publication date: 19.09.2018

Acta Protozoologica, 2018, Volume 57, Issue 1, pp. 49 - 52

https://doi.org/10.4467/16890027AP.18.004.8398

Authors

,
Inna V Grushevaya
All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia
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,
Anastasia N. Ignatieva
All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia
All publications →
,
Svetlana M. Malysh
All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia
All publications →
,
Igor V. Senderskiy
All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia
All publications →
,
Ivan V. Zubarev
All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia
All publications →
Anastasia G. Kononchuk
All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia
All publications →

Titles

Spore dimorphism in Nosema pyrausta (Microsporidia, Nosematidae): from morphological evidence to molecular genetic verification

Abstract

Microsporidia infection rate in Ostrinia nubilalis larvae collected in Russia in 2011-2016 ranged from 0 to 16 %. Totally, among 262 examined insects, there were as many as 13 infected specimens, resulting in average prevalence of 5 % over the period indicated. In all positive samples but one diplokaryotic spores 4 um long were observed corresponding to diagnosis of Nosema pyrausta. Nevertheless, in one case (i.e. 0.4 %) the infected larva contained monokaryotic spores about 2 um long. After experimental infection of a substitute host Ostrinia furnacalis with monokaryotic spores only Nosema-like spores were observed in laboratory assays. Ribosomal RNA and RPB1 gene portions were 100 % identical in samples of both mono- and diplokaryotic spores. This observation shows that Nosema pyrausta can form uninucleate spores under yet to be described conditions in nature and that molecular genetic analysis is essential for correct species identification. 

References

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Information

Information: Acta Protozoologica, 2018, Volume 57, Issue 1, pp. 49 - 52

Article type: Original article

Authors

All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia

All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia

All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia

All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia

All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia

All-Russian Institute for Plant Protection, Pushkin, Saint-Petersburg, Russia

Published at: 19.09.2018

Article status: Open

Licence: CC BY-NC-ND  licence icon

Percentage share of authors:

Inna V Grushevaya (Author) - 16%
Anastasia N. Ignatieva (Author) - 16%
Svetlana M. Malysh (Author) - 16%
Igor V. Senderskiy (Author) - 16%
Ivan V. Zubarev (Author) - 16%
Anastasia G. Kononchuk (Author) - 20%

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English