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Peculiarities of Trypanosoma rangeli KP1(-) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea,  Trypanosomatidae)

Publication date: 24.11.2017

Acta Protozoologica, 2017, Volume 56, Issue 2, pp. 119 - 127

https://doi.org/10.4467/16890027AP.17.010.7485

Authors

,
Maria A. Sousa
Laboratório de Toxoplasmose e Outras Protozooses, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil
All publications →
,
Barbara N. Santos
Coleção de Tripanossomatídeos
All publications →
,
Cristina Carvalhal
Coleção de Tripanossomatídeos
All publications →
Mario Steindel
Laboratório de Protozoologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil
All publications →

Titles

Peculiarities of Trypanosoma rangeli KP1(-) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea,  Trypanosomatidae)

Abstract

Two KP1(–) strains of Trypanosoma rangeli (SC-58, SC-61) isolated from the wild rodent Phyllomys dasythrix from Santa Catarina (Brazil) were compared with some KP1(+) reference stocks from different Latin America countries, and also with Trypanosoma lewisi. The strains were analyzed by some morphological and biological features, and by biochemical and molecular techniques. The mean total length (TL) of the bloodstream trypomastigotes of T. rangeli varied between 31.3–33.0 µm, and those of T. lewisi (adult forms) was 28.2 µm, values within the variation known for each species. In T. rangeli KP1(+) and T. lewisi, the nucleus was located in the anterior portion of the body, with nuclear indexes (NI) ≥ 1.2, as typically described for both species. Differently, most trypomastigotes of the KP1(–) stocks presented NI ≤ 1.0. Another striking feature of the KP1(–) strains was their very fastidiously growth in axenic cultures when compared with the KP1(+) stocks and T. lewisi. Three isoenzyme loci (MDH, IDH and PGM) clearly distinguished T. rangeli and T. lewisi, and the distinction between the KP1(+) and KP1(–) strains was possible at MDH, PGM and GPI loci. All T. rangeli strains presented the typical 760 bp amplicon derived from their KP2 minicircles. However, the KP3 products of the KP1(+) strains were a single large band (~330bp), whereas those of the KP1(–) had two distinct bands (350 and 300 bp). T. lewisi presented 700 and 400 bp amplicons, as previously reported. The peculiarities of T. rangeli isolates from P. dasythrix corroborate a possible speciation process within this taxon.

References

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Information

Information: Acta Protozoologica, 2017, Volume 56, Issue 2, pp. 119 - 127

Article type: Original article

Authors

Laboratório de Toxoplasmose e Outras Protozooses, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil

Coleção de Tripanossomatídeos

Coleção de Tripanossomatídeos

Laboratório de Protozoologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil

Published at: 24.11.2017

Article status: Open

Licence: CC BY-NC-ND  licence icon

Percentage share of authors:

Maria A. Sousa (Author) - 25%
Barbara N. Santos (Author) - 25%
Cristina Carvalhal (Author) - 25%
Mario Steindel (Author) - 25%

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English