Morphology, Ciliary Pattern and Molecular Phylogeny of Trachelophyllum brachypharynx Levander, 1894 (Litostomatea, Haptoria, Spathidiida)
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RIS BIB ENDNOTEMorphology, Ciliary Pattern and Molecular Phylogeny of Trachelophyllum brachypharynx Levander, 1894 (Litostomatea, Haptoria, Spathidiida)
Publication date: 17.02.2015
Acta Protozoologica, 2015, Volume 54, Issue 2, pp. 123 - 135
https://doi.org/10.4467/16890027AP.15.010.2735Authors
Morphology, Ciliary Pattern and Molecular Phylogeny of Trachelophyllum brachypharynx Levander, 1894 (Litostomatea, Haptoria, Spathidiida)
We isolated a relatively unknown haptorian ciliate, Trachelophyllum brachypharynx, in brackish water from the mouth of the Taehwa River, South Korea. The morphology of this isolate was studied using in vivo observation and protargol impregnation, and its evolutionary history was revealed by phylogenetic analysis of the 18S rRNA gene. The main features of T. brachypharynx include (i) a very narrowly fusiform and slightly contractile body about 380 × 40 μm in size; (ii) two ellipsoidal macronuclear nodules typically connected by a fine strand; (iii) a single terminal contractile vacuole; (iv) filiform extrusomes that are typically 30 µm long; (v) an average of 24 ciliary rows, with two of them anteriorly differentiated into an isostichad dikinetidal dorsal brush; and (vi) hat-shaped lepidosomes. Based on the 18S rRNA gene phylogeny, T. brachypharynx clustered together withTrachelophyllum sp. within the order Spathidiida. Furthermore, phylogenetic trees and networks indicate some members from the genera Enchelyodon and Spathidium as the nearest relatives of trachelophyllids. Therefore, based on the present molecular and comparative-morphological analyses, we suggested a hypothesis explaining how trachelophyllids may have evolved from a spathidiid-like ancestor via an enchelyodonid-like stage.
Castresana J. (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol. Biol. Evol. 17: 540–552
Chen Z., Song W. (2001) Phylogenetic positions of Uronychia transfuga and Diophrys appendiculata (Euplotida, Hypotrichia, Ciliophora) within hypotrichous ciliates inferred from the small ribosomal RNA gene sequences. Eur. J. Protistol. 37: 291–301
Coats D. W., Clamp J. C. (2009) Ciliated protists (Ciliophora) of the Gulf of Mexico. In: Gulf of Mexico: Origin, Waters, and Biota, Vol. 1. Biodiversity, (Eds. D. L. Felder, D. K. Camp). Texas A&M University Press, 57–79
Foissner W. (1984) Infraciliatur, Silberliniensystem und Biometrie einiger neuer und wenig bekannter terrestrischer, limnischer und mariner Ciliaten (Protozoa: Ciliophora) aus den Klassen Kinetofragminophora, Colpodea und Polyhymenophora. Stapfia 12: 1–165
Foissner W. (1994) Spetazoon australiense nov. gen., nov. spec., ein neues Wimpertier (Protozoa, Ciliophora) von Australien. Kataloge des OÖ. Landesmuseums (N. F.) 71: 267–278
Foissner W. (2005) Two new “flagship” ciliates (Protozoa, Ciliophora) from Venezuela: Sleighophrys pustulata and Luporinophrys micelae. Eur. J. Protistol. 41: 99–117
Foissner W., Xu K. (2007) Monograph of the Spathidiida (Ciliophora, Haptoria). Vol. I: Protospathidiidae, Arcuospathidiidae, Apertospathulidae. Springer-Verlag, Dordrecht
Foissner W., Berger H., Blatterer H., Kohmann F. (1995) Taxonomische und ökologische Revision der Ciliaten des Saprobiensystems – Band IV: Gymnostomatea, Loxodes, Suctoria. Informationsberichte des Bayerischen Landesamtes für Wasserwirtschaft, Deggendorf
Foissner W., Berger H., Schaumburg J. (1999) Identification and ecology of limnetic plankton ciliates. Informationsberichte des Bayerischen Landesamtes für Wasserwirtschaft, Deggendorf
Foissner W., Agatha S., Berger H. (2002) Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa) with emphasis on two contrasting environments, the Etosha region and the Namib Desert. Denisia 5: 1–1459
Grain J. (1994) Classe de Litostomatea. In: Infusoires Ciliés–Systematique, Traité de Zoologie, Vol. II, Fasc. 2, (Ed. P. de Puytorac). Masson, Paris, 267–310
Guindon S., Gascuel O. (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 52: 696–704
Guindon S., Dufayard J. F., Lefort V., Anisimova M., Hordijk W., Gascuel O. (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst. Biol. 59: 307–321
Huson D. H. (1998) SplitsTree: a program for analyzing and visualizing evolutionary data. Bioinformatics 14: 68–73
Huson D. H., Bryant D. (2006) Application of phylogenetic networks in evolutionary studies. Mol. Biol. Evol. 23: 254–267
International Commission on Zoological Nomenclature [ICZN] (1999) International Code of Zoological Nomenclature. 4th ed. Tipografia La Garangola, Padova
Kahl A. (1926) Neue und wenig bekannte Formen der holotrichen und heterotrichen Ciliaten. Arch. Protistenkd. 55: 197–438
Kahl A. (1930) Urtiere oder Protozoa I: Wimpertiere oder Ciliata (Infusoria) 1. Allgemeiner Teil und Prostomata. Tierwelt Dtl. 18: 1–180
Katoh K., Toh H. (2008) Recent developments in the MAFFT multiple sequence alignment program. Brief. Bioinform. 9: 286–298
Kwon C. B., Vďačný P., Shazib S. U. A., Shin M. K. (2014) Morphology and molecular phylogeny of a new haptorian ciliate, Chaenea mirabilis sp. n., with implications for the evolution of the dorsal brush in haptorians (Ciliophora, Litostomatea). J. Eukaryot. Microbiol. 61: 278–292
Levander K. M. (1894) Materialien zur Kenntnis der Wasserfauna in der Umgebung von Helsingfors, mit besonderer Berücksichtigung der Meeresfauna. I. Protozoa. Acta Soc. Fauna Flora fenn. 12: 1–115
Levander K. M. (1901) Übersicht der in der Umgebung von Esbo-Löfö im Meereswasser vorkommenden Thiere. Acta Soc. Fauna Flora fenn. 20: 1–20
Lipscomb D. L., Riordan G. P. (1990) The ultrastructure of Chaenea teres and an analysis of the phylogeny of the haptorid ciliates. J. Protozool. 37: 287–300
Medlin L., Elwood H. J., Stickel S., Sogin M. L. (1988) The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71: 491–499
Posada D. (2008) JModelTest: phylogenetic model averaging. Mol. Biol. Evol. 25: 1253–1256
Ronquist F., Huelsenbeck J. P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574
Shimodaira H. (2002) An approximately unbiased test of phylogenetic tree selection. Syst. Biol. 51: 492–508
Shimodaira H. (2008) Testing regions with non-smooth boundaries via multiscale bootstrap. J. Stat. Plan. Infer. 138: 1227–1241
Shimodaira H., Hasegawa M. (2001) Consel: for assessing the confidence of phylogenetic tree selection. Bioinformatics 17: 1246–1247
Swofford D. L. (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Ver. 4. Sinauer Associates, Sunderland, MA
Talavera G., Castresana J. (2007) Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst. Biol. 56: 564–577
Telesh I., Postel L., Heerkloss R., Mironova E., Skarlato S. (2009) Zooplankton of the open Baltic Sea: extended atlas. Meereswiss. Ber., Warnemünde 76: 1–290
Vďačný P., Foissner W. (2013) Synergistic effects of combining morphological and molecular data in resolving the phylogenetic position of Semispathidium (Ciliophora, Haptoria), with description of S. breviarmatum sp. n. from tropical Africa. Zool. Scr. 42: 529–549
Vďačný P., Bourland W. A., Orsi W., Epstein S. S., Foissner W. (2011) Phylogeny and classification of the Litostomatea (Protista, Ciliophora), with emphasis on free-living taxa and the 18S rRNA gene. Mol. Phylogenet. Evol. 59: 510–522
Vďačný P., Bourland W. A., Orsi W., Epstein S. S., Foissner W. (2012) Genealogical analyses of multiple loci of litostomatean ciliates (Protista, Ciliophora, Litostomatea). Mol. Phylogenet. Evol. 65: 397–411
Vďačný P., Breiner H.-W., Yashchenko V., Dunthorn M., Stoeck T., Foissner W. (2014) The chaos prevails: molecular phylogeny of the Haptoria (Ciliophora, Litostomatea). Protist 165: 93–111
Wilbert N. (1975) Eine verbesserte Technik der Protargolimprägnation für Ciliaten. Mikrokosmos 64: 171–179
Information: Acta Protozoologica, 2015, Volume 54, Issue 2, pp. 123 - 135
Article type: Original article
Ulsan University, Department of Biological Science, Ulsan, South Korea
Comenius University, Department of Zoology, Bratislava, Slovakia
Ulsan University, Department of Biological Science, Ulsan, South Korea
Ulsan University, Department of Biological Science, Ulsan, South Korea
Published at: 17.02.2015
Article status: Open
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