Acta Protozoologica

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Chlorophyll-a and Suspended Inorganic Material Affecting the Shell Traits
of Testate Amoebae Community

Publication date: 28.11.2016

Acta Protozoologica, 2016, Volume 55, Issue 3, pp. 145 - 154



Leilane Talita Fatoreto Schwind
Laboratory of Zooplankton, Nucleus of Research in Limnology, Ichthyology and Aquaculture (Nupélia), State University of Maringa, Maringa, PR, Brazil
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Rodrigo Leite Arrieira
Laboratory of Zooplankton, Nucleus of Research in Limnology, Ichthyology and Aquaculture (Nupélia), State University of Maringa, Maringa, PR, Brazil
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Claudia Costa Bonecker
Laboratory of Zooplankton, Nucleus of Research in Limnology, Ichthyology and Aquaculture (Nupélia), State University of Maringa, Maringa, PR, Brazil
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Fábio Amodêo Lansac-Tôha
Laboratory of Zooplankton, Nucleus of Research in Limnology, Ichthyology and Aquaculture (Nupélia), State University of Maringa, Maringa, PR, Brazil
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Shell composition is considered an important functional trait in testate amoebae community. Differences in the composition of shells may represent adaptations of these organisms to changes in the availability of environmental resources. We aimed to evaluate the influence of these environmental resources on the presence of different testate amoeba species, based on observation of one important functional trait (shell composition), in floodplain environments. We hypothesised that species diversity would increase with increased availability of food (chlorophyll-a; a critical environmental requirement). We also predicted that a higher concentration of suspended inorganic material would contribute to the occurrence of species that have an exogenous shell. Testate amoeba samples were taken between 2002 and 2011 in plankton of ten floodplain environments. A significant positive correlation was noted between the concentration of chlorophyll-a and the diversity of testate amoeba species according to a simple regression analysis. Furthermore, non-metric multidimensional scaling showed distinct groups with lower and higher concentration of suspended inorganic material. The occurrence of testate amoeba species with smaller exogenous shells was the most influenced by environments with higher concentrations of suspended inorganic material. Thus, our results emphasised that the evaluated environmental resources are important as assembly factors to shell traits in testate amoebae community.


Alves G. M., Velho L. F. M., Costa D. M., Lansac-Tôha F. A. (2012) Size structure in different habitats from a lake in the upper Paraná River floodplain. Eur. J. Protistol48: 169–177

Arrieira R. L., Schwind L. T. F., Bonecker C. C., Lansac-Tôha F. A. (2015a) Use of functional diversity to assess determinant assembly processes of testate amoebae community. Aquat. Ecol. 49: 561–571

Arrieira R. L., Alves G. M., Schwind L. T. F, Lansac-Tôha F. A. (2015b) Local factors affecting the testate amoeba community (Protozoa: Arcellinida; Euglyphida) in a neotropical floodplain. J. Limnol74: 444–452

Arrieira R. L., Schwind L. T. F., Bonecker C. C., Lansac-Tôha F. A. (2016) Environmental factors exert predominant effects on testate amoeba metacommunities during droughts in floodplains. Austral. Ecol. doi:10.1111/aec.12423

Auer B., Elzer U., Arndt H. (2004) Comparison of pelagic food webs in lakes along a trophic gradient and with seasonal aspects: influence of resource and predation. J. Plankton Res26: 697–709

Bastidas-Navarro M., Modenutti B. (2007) Efecto de la estructuración por macrófitas y por recursos alimentarios en la distribución horizontal de tecamebas y rotíferos en un lago andino patagónico. Rev. Chil. Hist. Nat. 80: 345–362

Beyens L., Meisterfeld R. (2001) Protozoa: testate amoebae. In: Smol J. P., Birks H. J., Last W. M. (Orgs.). Tracking Environment Change Using Lake Sediments. Terrestrial, Algal and Siliceous Indicators. Dordrecht: Kluwer Academic Publishers, 121–153

Bonnet L. (1976) Quelques aspects du peuplement thécamoebiens des sols des truffières. Protistologica 10: 281–289

Bottrell H. H., Duncan A., Gliwicz Z. M., Grygierek E., Herizing A., Hillbricht-Ilkowska A., Kurazawa H., Larsson P., Weglenska T. (1976) A review of some problems in zooplankton production studies. Norw. J. Zool24: 419–456

Chardez D. (1964) Sur la nutrition de Centropyxis discoides (Penard) Deflandre (Rhizopoda, Testacea). Bull. Rech. Agron. Gembloux3: 305–308

Charman D. J. (2001) Biostratigraphic and palaeoenvironmental applications of testate amoebae. Quat. Sci. Rev20: 1753–1764

Clarke K. R., Warwick R. M. (2001) Change in marine communities: an approach to statistical analysis and interpretation. Plymouth: PRIMER-E

Couteaux M. M., Pussard M. (1983) Nature du régime alimentaire des Protozoaires du sol. In: Lebrun P., André H. M., De Medts A., 
Grégoire-Wibo C., Wauthy G. (Eds) New Trends in Soil Biology. Belgium: Proceedings of the VIII. Int. Colloquium of Soil Biology, 179–195

Dalby A. P., Arun K., Moore J. M., Patterson R. T. (2000) Preliminary survey of arcellaceans (thecamoebians) as limnological indicators in tropical lake Sentani, Irian Jaya, Indonesia. J. Foramin. Res30: 135–142

du Châtelet E. A., Guillot F., Recourt P., Ventalon S., Tribovillard N. (2010) Influence of sediment grain size and mineralogy on testate amoebae test construction. C. R. Geosci. 342: 710–717

du Châtelet E. A., Bernard N., Delaine M., Potdevin J., Gilbert D. (2015) The mineral composition of the tests of ‘testate amoebae’ (Amoebozoa, Arcellinida): The relative importance of grain availability and grain selection. Revue de Micropaléontologie 58: 141–154

Gilbert D., Amblardb C., Bourdierb G., Francez A. J., Mitchell A. D. 
(2000) Le régime alimentaire des Thécamoebiens (Protista, Sarcodina). Année. Biol. 39: 57–68

Gilbert D., Mitchell A. D., Amblardb C., Bourdierb G., Francez A. J. (2003) Population dynamics and food preferences of the testate amoebae Nebela tincta major-bohemica-collaris complex (Protozoa) in a Sphagnum peatland. Acta Protozool. 42: 99–104

Gimenes M. F., Benedito-Cecilio E., Takeda A. M., Vismara M. R. (2004) Availability of sedimentary organic matter for benthic fishes of the upper Paraná river floodplain. Acta Sci. Biol. Sci. 26: 181–187

Golterman H. L., Clyno R. S., Ohnstad M. A. M. (1978) Methods for physical and chemical analysis of freshwaters. Oxford: Blackwell

Lansac-Tôha F. A., Bonecker C. C., Velho L. F. M., Simões N. R., Dias J. D., Alves G. M., Takahashi E. M. (2009) Biodiversity of zooplankton communities in the upper Paraná river floodplain: interannual variation from long-term studies. Braz. J. Biol. 69: 539–549

Lansac-Tôha F. A, Velho L. F. M., Costa D. M., Simões N. R., Alves G. M. (2014) Structure of the testate amoebae community in different habitats in a neotropical floodplain. Braz. J. Biol74: 181–190

Legendre P., Legendre L. (1998) Numerical ecology. Amsterdam, Elsevier Science Ltd.

McCune B., Grace J. B., Urban D. L. (2002) Analysis of ecological communities. Gleneden Beach: OR MjM software design

Medioli F. S., Scott D. B. (1983) Holocene Arcellacea (Thecamoebians) from Eastern Canada. Cushman Found. Foram. Res. Spec. Pub21: 5–63

Medioli F. S., Scott D. B., Abbott B. H. (1987) A case study of protozoan intraclonal variability: taxonomic implications. J. Foramin. Res17: 28–47

Mieczan T. (2012). Distribution of testate amoebae and ciliates in different types of peatlands and their contributions to the nutrient supply. Zool. Stud51: 18–26

Mitchell E. A. D., Payne R. J., Lamentowicz M. (2008) Potential implications of differential preservation of testate amoeba shells for paleoenvironmental reconstruction in peatlands. J. Paleolimnol. 40: 603–618.

Naeem S., Wright J. P. (2003) Disentangling biodiversity effects on ecosystem functioning: deriving solutions to a seemingly insurmountable problem. Ecol. Lett. 6: 567–579

Neiff J. J. (1996) Large rivers of South America: toward the new approach. Verh. Int. Ver. Limnol. 26: 167–180

Ogden C. G. (1989) The agglutinate shell of Heleopera petricola (Protozoa, Rhizopoda), factors affecting its structure and composition. Arch. Protistenkd37: 9–24

Pielou E. C. (1975) Ecological diversity. New York, John Wiley & Sons.

R Core Team (2012) R: Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna. ISBN 3-900051-07-0, URL http://www.R-project.org/

Reid G. K. (1961) Ecology of Inland Waters and Estuaries. New York: Reinhold

Rodriguez-Zaragoza S. (1994) Ecology of free-living amoebae. Crit. Rev. Microbiol. 20: 225–241

Schwind L. T. F., Arrieira R. L., Mantovano T., Bonecker C. C., Lansac-Tôha F. A. (2016a) Temporal influence on the functional traits of testate amoebae in a floodplain lake. Limnetica (in press).

Schwind L. T. F., Arrieira R. L., Dias J. D., Simões N.R., Bonecker, C. C., Lansac-Tôha F. A. (2016b) Effect of daily variation on the seasonality of testate amoebae community structure in different environments of a neotropical floodplain. J. Limnol.75: 78–89

Scott D. B., Medioli F. S., Schafer C. T. (2001) Monitoring in coastal environments using foraminifera and thecoamoebian indicators. Cambridge: Cambridge University Press

Simões N. R., Dias J. D., Leal C. M., Braghin L. S. M., Lansac-Tôha F. A., Bonecker C. C. (2013) Floods control the influence of environmental gradients on the diversity of zooplankton communities in a neotropical floodplain. Aq. Sci75: 607–617

Sokal R. R., Rolhf F. J. (1991) Biometry: the Principles and Practice of Statistics in Biological Research. New York: WH Freeman and Company

Souza M. B. G. (2008) Guia das Tecamebas. Bacia do rio Peruaçu – Minas Gerais. Subsídio para conservação e monitoramento da Bacia do Rio São Francisco. Belo Horizonte: Editora UFMG

Statsoft Inc. (2005) Statistica for Windows (data analysis software system), version 7.1. Tulsa: Statsoft Inc.

Torres V. S., Jebram D. H. A. (1993) Arcella gibbosa microsoma var. n. (Protozoa: Sarcodina, Arcellinida) Descrição e Observações Feitas em seu Cultivo. Biotemas 6: 20–29

Velho L. F. M., Lansac-Tôha F. A., Bonecker C. C., Zimmermann-Callegari M. C. (2000) On the occurrence of testate amoebae (Protozoa, Rhizopoda) in Brazilian inland waters. II. Families Centropyxidae, Trigonopyxidae and Plagiopyxidae. Acta Sci. Biol. Sci. 22: 365–374

Velho L. F. M., Lansac-Tôha F. A., Bonecker C. C., Zimmermann-Callegari M. C., Aoyagui A. S. M. (2001) On the occurrence of testate amoebae (Protozoa, Amoebozoa, Rhizopoda) in Brazilian inland waters. IV. Families Difflugiidae (genera Cucurbitella, LagenodifflugiaPentagoniaPontigulasiaProtocucurbitellaSuiadifflugia) and Lesquereusiidae (genera LesquereusiaQuadrulellaNetzelia). Acta Sci. Biol. Sci23: 323–332

Violle C., Navas M. L., Vile D., Kazakou E., Fortunel C., Hummel I., Garnier E. (2007) Let the concept of trait be functional! Oikos 116: 882–892

Wetzel R. G., Liken G. E. (1991) Limnological Analyses. New York: Springer-Verlag


Information: Acta Protozoologica, 2016, pp. 145 - 154

Article type: Original research article


Laboratory of Zooplankton, Nucleus of Research in Limnology, Ichthyology and Aquaculture (Nupélia), State University of Maringa, Maringa, PR, Brazil

Laboratory of Zooplankton, Nucleus of Research in Limnology, Ichthyology and Aquaculture (Nupélia), State University of Maringa, Maringa, PR, Brazil

Laboratory of Zooplankton, Nucleus of Research in Limnology, Ichthyology and Aquaculture (Nupélia), State University of Maringa, Maringa, PR, Brazil

Laboratory of Zooplankton, Nucleus of Research in Limnology, Ichthyology and Aquaculture (Nupélia), State University of Maringa, Maringa, PR, Brazil

Published at: 28.11.2016

Article status: Open

Licence: None

Percentage share of authors:

Leilane Talita Fatoreto Schwind (Author) - 25%
Rodrigo Leite Arrieira (Author) - 25%
Claudia Costa Bonecker (Author) - 25%
Fábio Amodêo Lansac-Tôha (Author) - 25%

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