FAQ
Jagiellonian University logo

Cap Formation Induced by Concanavalin A in Pathogenic Free-living Amoebae

Publication date: 15.05.2011

Acta Protozoologica, 2011, Volume 50, Issue 2, pp. 81 - 87

https://doi.org/10.4467/16890027AP.11.010.0010

Authors

,
Arturo González-Robles
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados, México DF, México
All publications →
,
A. R. Cristóbal-Ramos
All publications →
,
Monica González-Lázaro
https://orcid.org/0000-0003-3878-5782 Orcid
All publications →
,
M. Omaña-Molina
All publications →
Adolfo Martínez-Palomo
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados, México DF, México
All publications →

Titles

Cap Formation Induced by Concanavalin A in Pathogenic Free-living Amoebae

Abstract

Morphological differences in cap formation were found when trophozoites of different free-living amoebae were treated with the lectin Concanavalin A, which resulted in a rapid redistribution of certain surface components to form small clusters and membrane-folded structures of diverse sizes. Acanthamoeba castellanii, Acanthamoba polyphaga and Naegleria lovaniensis exhibited characteristic caps, however, in A. castellanii this structure was larger and included several folds of the plasma membrane; furthermore, some of these caps had vacuoles containing a fi bro granular content. In contrast, the caps formed by A. polyphaga and N. lovaniensis lacked vacuoles. Regarding Naegleria fowleri, the trophozoites did not produce a defi ned cap, and only small patches of lectin-bound surface receptor complexes were observed at one pole of the cell body. In the free-living amoebae studied, it was not possible to correlate the shape and size of cap with pathogenicity.

References

Download references

Aust-Kettis A., Sundqvist K. G. (1978) Dynamics of the interaction between Entamoeba histolytica and components of the immune response. Scand. J. Parasitol7: 35–44

Barry J. D. (1979) Capping of variable antigen on Trypanosoma brucei, and its immunological and biological significance. J. Cell Sci. 37: 287–302

Bourguignon L. Y. W., Bourguignon G. J. (1984) Capping and the cytoskeleton. Int. Rev. Cytot. 87: 195–224

Calderón J., Muñoz L., Acosta H. (1980) Surface redistribution and release of antibody-induced caps in Entamoebae. J. Exp. Med151: 184–193

Calderón J., Avila E. E. (1986) Antibody-induced caps in Entamoeba histolityca: isolation and electrophoretic analysis. J. Infect. Dis. 153: 927–932

Clark D. W., Niederkorn J. Y. (2006) The pathophysiology of Acanthamoeba keratitis. Trends Parasitol. 22: 175–180

Cogo P. E., Scaglia M., Gatti S., Rossetti F., Alaggio R., Laverda A. M., Zhou L., Xiao L., Visvesvara G. (2004) Fatal Naegleria fowleri meningoencephalitis, Italy. Emerg. Infect. Dis. 10: 1835–1837

Cooper J. A., Blum J. D., Pollard T. D. (1984) Acanthamoeba castellani capping protein: properties, mechanism of action, immunologic cross-reactivity, and localization. J. Cell Biol. 99: 217–225

Cursons R., Sleigh J., Hood D., Pullon D. (2003) A case of primary amoebic meningoencephalitis: North Island, New Zealand. New Zeal. Med. J. 116: U712

Doyle J. J., Behin R., Mauel J., Rowe D. S. (1974) Antibody-induced movement of membrane components of Leishmania enriettii. J. Exp. Med. 139: 1061–1069

Dwyer D. M. (1976) Antibody-induced modulation of Leishmania donovani surface membrane antigens. J. Immunol. 117: 2081– 2091

Dzbenski T. H., Michalak T., Plonka W. S. (1976) Electron microscopic and radioisotopic studies on cap formation in Toxoplasma gondiiInfect. Immun. 14: 1196–1201

Espinosa-Cantellano M., Martínez-Palomo A. (1994) Entamoeba histolytica: Mechanism of Surface Receptor Capping. Exp. Parasitol. 79: 424–435

Ferrante A., Thong Y. H. (1979) Antibody induced capping and endocytosis of surface antigens in Naegleria fowleri. Int. J. Parasitol9: 599–601

González-Robles A., Castañón G., Cristóbal-Ramos A. R., Hernández-Ramírez V. I., Omaña-Molina M., Martínez-Palomo A. (2007) Cell surface differences of Naegleria fowleri and Naegleria lovaniensis exposed with surface markers. Exp. Parasitol. 117: 399–404

Gun I., Paatero L., Wilkström L., Isomaa B. (1988) Concanavalin A-induced redistribution of surface receptors in Acanthamoeba castellanii at different growth phases. Eur. J. Cell Biol. 47: 112–120

Holifield B. F., Ishihara A., Jacobson K. (1990) Comparative behavior of membrane-protein complexes in motile fibroblasts: implications for a mechanism of capping. J. Cell Biol. 111: 2499–2512

John D. T. (1993) Opportunistically pathogenic free-living amebae. In: Parasitic Protozoa, (Eds. J. P. Kreier, J. R. Baker). 2nd ed., Vol. 3. Academic Press, San Diego

Khan N. A. (2009) In: Acanthamoeba Biology and Pathogenesis. Caister Academic Press, Norfolh, UK

King G. A., Preston T. M. (1977) Studies of anionic sites on the cell surface of the amoebae Naegleria gruberi using cationized ferritin. J. Cell Sci. 28: 133–149

Kwiatkowska K., Sobota A. (1997) Local accumulation of α-spectrin-related protein under plasma membrane during capping and phagocytosis in Acanthamoeba. Cell Motil. Cytoskel36: 253–265

Lawande R.V., Macfarlane T. J., Weir W. R. C., Awunor-Renner C. (1980) A case of primary amebic meningoencephalitis in a Nigerian farmer. Am. J. Trop. Med. Hyg. 29: 21–25

Ma P., Visvesvara G. S., Martinez A. J., Theodore F. H., Daggett P. M., Sawyer T. K. (1990) Naegleria and Acanthamoeba infections: Review. Rev. Infect. Dis. 12: 490–513

Martinez A. J. (1980) Is Acanthamoeba encephalitis an opportunistic infection? Neurology 30: 567–574

Martinez A. J. (1991) Infection of the central nervous system. Rev. Infect. Dis. 15 (Suppl 5)S399–S402

Martinez A. J. (1993) Free-living amebas: Infection of the central nervous system. Mt. Sin. J. Med60: 271–278

Martinez A. J., Visvesvara G. S. (1997) Free-living, amphizoic and opportunistic amebas. Brain Pathol. 7: 583–598

Martínez-Palomo A., González-Robles A. (1973) Selective agglutination of pathogenic strains of Enthamoeba histolytica induced con A. Nat. New Biol. 245: 186–187

Martínez-Palomo A., de Souza W., González-Robles A. (1976) Topographical differences in the distribution of surface coat components and intramembrane particles. A cytochemical and freeze-fracture study in culture forms of Trypanosoma cruziJ. Cell Biol. 69: 507–513

Martínez-Palomo A. (1982) The Biology of Entamoeba histolytica. In: Tropical Medicine Research Studies Series (Ed. K. N. Brown), Research Studies Press. John Wiley & Sons Ltd

Pinto da Silva P., Martínez-Palomo A., González-Robles A. (1975) Membrane structure and surface coat of Entamoeba histolityca. J. Cell Biol. 64: 538–550

Preston T. M., King C. A. (1984) Binding sites for bacterial flagella at the surface of the soil amoeba Acanthamoeba. J. Gen. Microbiol. 130: 1449–1458

Ray D. L. (1951) Agglutination of bacteria: a feeding method in the soil amoeba Hartmanella sp. J. Exp. Zool. 118: 443–465

Rivera F., Lares F., Ramirez E., Bonilla P., Rodríguez S., Labastida A., Ortíz R., Hernández D. (1991) Pathogenic Acanthamoeba isolated during an atmospheric survey in Mexico City. Rev. Infect. Dis. 13: S388–S389

Schaumberg D. A., Snow K. K., Dana M. R. (1998) The epidemic of Acanthamoeba keratitis: where do we stand? Cornea 17: 3–10

Schmuñis G. A., Szarfman A., De Souza W., Langembach T. (1980) Trypanosoma cruzi: Antibody induced mobility of surface antigens. Exp. Parasitol. 50: 90–102

Schuster F. L., Visvesvara G. S. (2004) Free-living amoebae as opportunistic pathogens of humans and animals. Int. J. Parasitol. 34: 1001–1027

Tavares P., Sansonetti P., Guillén N. (2000) The interplay between receptor capping and cytoskeleton remodeling in Entamoeba histoltycaArch. Med. Res. 31: S140–S142

Taylor R. B., Duffus W. P. H., Raff M. C., de Petris S. (1971) Redistribution and pinocytosis of lymphocyte surface immunoglobulin antibody. Nat. New Biol. 23: 225–229

Trissl D., Martínez-Palomo A., Argüello C., de la Torre M., de la Hoz R. (1977) Surface properties related to Concanavalin Ainduced agglutination. J. Exp. Med. 145: 652–665

Visvesvara G. S., Stehr-Green J. K. (1990) Epidemiology of freeliving ameba infections. J. Protozool. 37: 25S–33S

Walker G., Kerrick W. G., Bourgignon L. Y. (1989) The role of caldesmon in the regulation of receptor capping in mouse T-lymphoma cell. J. Biol. Chem. 264: 496–500

Information

Information: Acta Protozoologica, 2011, Volume 50, Issue 2, pp. 81 - 87

Article type: Original article

Authors

Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados, México DF, México

Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados, México DF, México

Published at: 15.05.2011

Article status: Open

Licence: None

Percentage share of authors:

Arturo González-Robles (Author) - 20%
A. R. Cristóbal-Ramos (Author) - 20%
Monica González-Lázaro (Author) - 20%
M. Omaña-Molina (Author) - 20%
Adolfo Martínez-Palomo (Author) - 20%

Article corrections:

-

Publication languages:

English