A New Species of Flamella (Amoebozoa, Variosea, Gracilipodida) Isolated from a Freshwater Pool in Southern Mississippi, USA

Austin C. Walthall; Alexander K. Tice; Matthew W.  Brown

A New Species of Flamella (Amoebozoa, Variosea, Gracilipodida) Isolated from a Freshwater Pool in Southern Mississippi, USA

Publication date: 25.08.2016

Acta Protozoologica, 2016, Volume 55, Issue 2, pp. 111 - 117

https://doi.org/10.4467/16890027AP.16.010.4945

Authors

Pobierz pełny tekst

PDF

Titles

A New Species of Flamella (Amoebozoa, Variosea, Gracilipodida) Isolated from a Freshwater Pool in Southern Mississippi, USA

Abstract

We isolated and identified a freshwater amoebozoan species that belongs to the genus Flamella Schaeffer, 1926 by single cell isolation and light microscopy. Our specific strain was isolated from a water sample obtained on the cover of a swimming pool in Petal, Mississippi, USA collected during the winter of 2015. Morphologically, our isolate is a fan-shaped amoeba with a large, frontal hyaloplasm and distinctive granuloplasm. It is capable of encystment and trophozoites occasionally have two nuclei. The isolate (GFP151sc) is phylogenetically sister to but unique from the freshwater environmental flamellid clone from Borok, Yaroslavl region, Russia originally published in 2006. Here we describe and place this isolate into a new species, Flamella piscinae n. sp.

Keywords

Amoeboid, amoeba, SSU rDNA, taxonomy, protist, phylogenetics, Flamella, Gracilipoida

References

Download references

Adl S. M., Simpson A. G., Lane C. E., Lukes J., Bass D., Bowser S. S., Brown M. W., Burki F., Dunthorn M., Hampl V., Heiss A., Hoppenrath M., Lara E., Le Gall L., Lynn D. H., McManus H., Mitchell E. A., Mozley-Stanridge S. E., Parfrey L. W., Pawlowski J., Rueckert S., Shadwick R. S., Schoch C. L., Smirnov A., Spiegel F. W. (2012) The revised classification of eukaryotes. J. Eukaryot. Microbiol. 59: 429–493

Bovee E. C. (1956) Some observations on the morphology and activities of new ameba from citrus wastes, Flamella citrensis n. sp. J. Protozool. 3: 151–153

Berney C., Geisen S., Van Wichelen J., Nitsche F., Vanormelingen P., Bonkowski M., Bass D. (2015) Expansion of the ‘Reticulosphere’: Diversity of Novel Branching and Network-forming Amoebae Helps to Define Variosea (Amoebozoa). Protist. 166: 271–295

Brown M. W., Silberman J. D., Spiegel F. W. (2012) A contemporary evaluation of the acrasids (Acrasidae, Heterolobosea, Excavata). Eur. J. Protistol. 48: 103–123

Cavalier-Smith T., Chao E.-Y., Oates B. (2004) Molecular phylogeny of Amoebozoa and evolutionary significance of the unikont Phalansterium. Eur. J. Protistol. 40: 21–48

Dykova I., Kostka M. (2013) Illustrated Guide to Culture Collection of Free-Living Amoebae. Academia, Prague, Czech Republic.

Fiore-Donno A. M., Meyer M., Baldauf S. L., Pawlowski J. (2007) Evolution of dark-spored Myxomycetes (slime-molds), molecules versus morphology. Mol. Phylogenet. Evol. 46: 787–889

Fishbeck D. W., Bovee E. C. (1993) Two new amoebae, Striamoeba sparolata n. sp. and Flamella tiara n. sp., from fresh water. Ohio J. Sci. 93: 134–139

Kudryavtsev A., Wylezich C., Schlegel M., Walochnik J., Michel R. (2009) Ultrastructure, SSU rRNA Gene Sequences and Phylogenetic Relationships of Flamella Schaeffer, 1926 (Amoebozoa), with Description of Three New Species. Protist. 160: 21–40

Lahr D. J. G., Grant J., Nguyen T., Lin J. H., Katz L. A. (2011) Comprehensive Phylogenetic Reconstruction of Amoebozoa Based on Concatenated Analyses of SSU-rDNA and Actin Genes. PLoS One. 6: e22780

Lahr D. J. G., Anderson O. R., Kubik G. M., Grant J., Gant A. L., Katz L.A. (2012) Morphological Description of Telaepolella tubasferens n. g., n. sp., Isolate ATCC 50593, a Filose Amoeba in the Gracilipodida, Amoebozoa. Acta Protozool. 51: 305–318

Maddison W. P., Maddison D. R. (2015) Mesquite: a modular system for evolutionary analysis. Version 3.04 http://mesquiteproject.org

Michel R., Smirnov A. V. (1999) The Genus Flamella Schaeffer, 1926 (Lobosea, Gymnamoebia), with a Description of Two New Species. Eur. J. Protistol. 35: 403–410

Nikolaev S. I., Berney C., Petrov N. B., Mylnikov A. P., Fahrni J. F., Pawlowski J. (2006) Phylogenetic position of Multicilia marina and the evolution of Amoebozoa. Int. J. Syst. Evol. Microbiol. 56: 1449–1458

Page F. C. (1988) A New Key to Freshwater and Soil Gymnamoebae. Freshwater Biological Association, Ambleside, Cumbria, UK

Posada D., Crandall K. A. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817–818

Ronquist F., Huelsenbeck J. P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574

Schaeffer A. A. (1926) Taxonomy of the Amoebas. Papers Dept. Mar. Biol. Carnegi. Inst. Washington USA. 24: 3–112

Shmakova L., Bondarenko N., Smirnov A. (2016) Viable species of Flamella (Amoebozoa: Variosea) isolated from ancient Arctic permafrost sediments. Protist. 167: 13–30

Smirnov A. V., Chao E., Nassonova E. S., Cavalier-Smith T. (2011) A Revised Classification of Naked Lobose Amoebae (Amoebozoa: Lobosa). Protist. 162: 545–570

Stamatakis A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313

Stock A., Jürgens K., Bunge J., Stoeck T. (2009) Protistan diversity in suboxic and anoxic waters of the Gotland Deep (Baltic Sea) as revealed by 18S rRNA clone libraries. Aquat. Microb. Ecol. 55: 267–284

Tekle Y. I., Grant J., Anderson O. R., Nerad T. A., Cole J. C., Patterson D. J., Katz L. A. (2008) Phylogenetic placement of diverse amoeba inferred from multigene analyses and assessment of clade stability within ‘Amoebozoa’ upon removal of varying rate classes of SSU-rDNA. Mol. Phylogent. Evol. 47: 339–352

Watson P. M., Sorrell S. C., Brown M. W. (2014) Ptolemeba n. gen., a Novel Genus of Hartmanellid Amoebae (Tubulinea, Amoebozoa); with an Emphasis on the Taxonomy of Saccamoeba. J. Eukaryot. Microbiol. 61: 611–619

Information

Information: Acta Protozoologica, 2016, Volume 55, Issue 2, pp. 111 - 117

DOI: https://doi.org/10.4467/16890027AP.16.010.4945

Article type: Original article

Authors

Austin C. Walthall

Department of Biological Sciences, Mississippi State University, Mississippi State, USA

Alexander K. Tice

Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, USA

Matthew W. Brown

Institute for Genomics, Biocomputing & Biotechnology, Mississippi State University, Mississippi State, USA

Published at: 25.08.2016

Article status: Open

Licence: None

Percentage share of authors:

Austin C. Walthall (Author) - 33%

Alexander K. Tice (Author) - 33%

Matthew W. Brown (Author) - 34%

Article corrections:

Publication languages:

English

View count: 3139

Number of downloads: 1567

A New Species of Flamella (Amoebozoa, Variosea, Gracilipodida) Isolated from a Freshwater Pool in Southern Mississippi, USA

Download xml