In the paper, the analysis of the chosen parameters effects (gas flow rate, the impeller speed, type of impeller and concentration of the aqueous solutions of sucrose) on the gas hold-up for gas-liquid and gas-liquid--biophase systems mechanically agitated are presented. Experimental studies were conducted in a vessel of the diameter of 0.288 m. The agitated vessel was equipped with Rushton or A 315 impeller. The results were described mathematically. The study shows that gas hold-up strongly depends on the impeller speed and the gas flow rate.