%0 Journal Article
%T Occurrence of New Polyenoic Very Long Chain Acyl Residues in Lipids from Acanthamoeba castellanii
%A Palusińska-Szysz, Marta
%A Turska-Szewczuk, Anna
%A Karaś, Magdalena
%A Russa, Ryszard
%A Drożański, Wincenty J.
%J Acta Protozoologica
%V 2009
%N Volume 48, Issue 1
%P 63-72
%K Acanthamoeba castellanii; endoparasites, long chain polyenoic fatty acids, double bond position, dimethyloxazoline derivatives, dimetyldisulfide adducts, non- methylene interrupted fatty acids
%@ 0065-1583
%D 2009
%U https://ejournals.eu/en/journal/acta-protozoologica/article/occurrence-of-new-polyenoic-very-long-chain-acyl-residues-in-lipids-from-acanthamoeba-castellanii
%X The cellular fatty acid composition of Acanthamoeba castellanii, a unicellular bacteriovorous organism, was reinvestigated. Lipids from amoebae grown axenically in proteose peptone-yeast extract-glucose medium were extracted with chloroform–methanol and separated by silicic acid column chromatography into non-polar and polar fractions. The fatty acid composition of the lipids and the double-bond position of the unsaturated acids have been determined by capillary gas chromatography-mass spectrometry (GC-MS) of their corresponding methyl esters, 2-alkenyl-4,4-dimethyloxazoline (DMOX) derivatives and dimethyldisulfide (DMDS) adducts. Evidence is given that lipids from A. castellanii in addition to the three already identified saturated straight chain fatty acids: tetradecanoic (C14:0), hexadecanoic (C16:0), octadecanoic (C18:0), and six preponderant unsaturated fatty acids: hexadecenoic (C16:1 Δ7), octadecenoic (C18:1 Δ9), octadecadienoic (C18:2 Δ9,12), eicosadienoic (C20:2 Δ11,14), eicosatrienoic (C20:3 Δ8,11,14), and eicosatetraenoic (C20:4 Δ5,8,11,14), contain additionally four very long chain unsaturated fatty acids: octacosenoic (C28:1 Δ21), octacosadienoic (C28:2 Δ5,21), triacontadienoic (30:2 Δ21,24), and triacontatrienoic (C30:3 Δ5,21,24) previously unreported in lipids of A. castellanii. These new long chain fatty acids account for approximately 25% of total fatty acids. To our knowledge, this is the first report of very long chain polyenoic fatty acids present in lipids extracted from A. castellanii cells.