TY - JOUR
TI - Tubulinosema loxostegi sp. n. (Microsporidia: Tubulinosematidae) from the Beet Webworm Loxostege sticticalis L. (Lepidoptera: Crambidae) in Western Siberia
AU - Malysh, Julia M.
AU - Tokarev, Yuri S.
AU - Sitnicova, Natalia V.
AU - Martemyanov, Vyacheslav V.
AU - Frolov, Andrei N.
AU - Issi, Irma V.
TI - Tubulinosema loxostegi sp. n. (Microsporidia: Tubulinosematidae) from the Beet Webworm Loxostege sticticalis L. (Lepidoptera: Crambidae) in Western Siberia
AB - Adults of beet webworm Loxostege sticticalis were collected in Western Siberia in 2009 and 2010. A microsporidium was found infecting 12 of 50 moths in 2010. The parasite develops in direct contact with host cell cytoplasm, sporogony is presumably disporoblastic. The spores are ovoid, diplokaryotic, 4.2 × 2.4 μm in size (fresh), without a sporophorous vesicle. Electron microscopy showed: (a) tubules on the surface of sporoblasts and immature spores; (b) slightly anisofilar polar tube with 10–14 coils, last 2–3 coils of lesser electron density; (c) bipartite polaroplast with anterior and posterior parts composed of thin and thick lamellae, respectively; (d) an indentation in the region of the anchoring disc; (e) an additional layer of electron-dense amorphous matter on the exospore surface. The spore ultrastructure is characteristic of the genus Tubulinosema. Sequencing of small subunit and large subunit ribosomal RNA genes showed 98–99.6% similarity of this parasite to the Tubulinosema species available on Genbank. A new species Tubulinosema loxostegi sp. n. is established.
VL - 2013
IS - Volume 52, Issue 4
PY - 2014
SN - 0065-1583
C1 - 1689-0027
SP - 299
EP - 308
DO - 10.4467/16890027AP.13.028.1319
UR - https://ejournals.eu/en/journal/acta-protozoologica/article/tubulinosema-loxostegi-sp-n-microsporidia-tubulinosematidae-from-the-beet-webworm-loxostege-sticticalis-l-lepidoptera-crambidae-in-western-siberia
KW - Beet webworm
KW - microsporidia
KW - taxonomy
KW - molecular phylogenetics
KW - Tubulinosema