TY - JOUR
TI - Improvement of Silver Impregnation Technique Using in Situ Synthesized Protargol
AU - Kurilov, Alexander V.
TI - Improvement of Silver Impregnation Technique Using in Situ Synthesized Protargol
AB - An improved impregnation method for ciliates has been described with a new formulation for silver proteinate synthesized in situ, that avoids necessary its long-time laboratory synthesis or use of expensive commercial protargol. Compared to conventional techniques, the proposed protocol is more time-saving, reduces the consumption of chemicals and excludes some hazardous ones (e.g. xylene). Structures that are impregnated such as nuclear apparatus, infraciliature, cortical and cytoplasmic microtubules are stained almost identical compared to other protargol methods. Advantages of this method allow us to merge it successfully with ecological quantitative studies of various natural communities of ciliates and provide correct identification of species during such investigations.
VL - 2017
IS - Volume 56, Issue 2
PY - 2017
SN - 0065-1583
C1 - 1689-0027
SP - 109
EP - 118
DO - 10.4467/16890027AP.17.009.7484
UR - https://ejournals.eu/en/journal/acta-protozoologica/article/improvement-of-silver-impregnation-technique-using-in-situ-synthesized-protargol
KW - ciliates
KW - in situ synthesized protargol technique
KW - staining method