TY - JOUR
TI - Surface Coat Differences between Invasive Entamoeba histolytica and Non-Invasive Entamoeba dispar
AU - González-Robles, Arturo
AU - Chávez-Munguía, Bibiana
AU - Salazar‑Villatoro, Lizbeth
AU - Lagunes-Guillén, Anel
AU - Hernández-Ramírez, Verónica Ivonne
AU - Talamás-Rohana, Patricia
AU - Martínez-Palomo, Adolfo
TI - Surface Coat Differences between Invasive Entamoeba histolytica and Non-Invasive Entamoeba dispar
AB - Using ultrastructural cytochemical techniques we have found differences in the distribution of surface coat components between the invasive protozoan parasite Entamoeba histolytica and the non-invasive Entamoeba dispar. Carbohydrate-containing components and anionic sites in the cell surface of both species were detected by staining with ruthenium red and cationized ferritin, respectively. Ruthenium red staining revealed a thicker surface coat in E. histolytica trophozoites, whereas trophozoites of E. dispar showed a higher concentration of cationized ferritin particles on its surface. Mannose or glucose residues were found at the plasma membrane of both parasites treated with Concanavalin A (Con A)-peroxidase; the surface reaction product was more evident in E. dispar, compared with E. histolytica. Con A rapidly produced surface caps in E. histolytica trophozoites, whereas E. dispar showed a much less efficient mobilization of surface Con A receptors. Agglutination with Con A produced much larger clumps  in E. histolytica in comparison with E. dispar. In turn, biotinylation assays revealed striking differences in the composition of surface membrane proteins in both amebic species. Overall, these results further emphasize the phenotypic differences between these two  common parasites of the human intestinal tract, once considered to be the same protozoan.
VL - 2015
IS - Volume 54, Issue 3
PY - 2015
SN - 0065-1583
C1 - 1689-0027
SP - 209
EP - 218
DO - 10.4467/16890027AP.15.017.3214
UR - https://ejournals.eu/en/journal/acta-protozoologica/article/surface-coat-differences-between-invasive-entamoeba-histolytica-and-non-invasive-entamoeba-dispar
KW - Amebiasis
KW - Entamoeba histolytica
KW - Entamoeba dispar
KW - Cell surface
KW - Concanavalin A.