@article{178b9d84-5d64-438e-bebf-1154e63f3255,
author = {Daniel Rodrigues-Silva, Celso Vataru Nakamura, Benedito Prado Dias Filho, Tânia Ueda-Nakamura, Diógenes Aparício Garcia Cortez},
title = {In vitro Antileishmanial Activity of Hydroalcoholic Extract, Fractions, and Compounds Isolated from Leaves of Piper ovatum Vahl against Leishmania amazonensis},
journal = {Acta Protozoologica},
volume = {2009},
number = {Volume 48, Issue 1},
year = {2009},
issn = {0065-1583},
pages = {73-81},keywords = {Antiprotozoan activity; ultrastructure alterations; Leishmania amazonensis; Piper ovatum},
abstract = {We assessed the biological activity of a crude extract, a mixture of several fractions, and a pure compound obtained from Piper ovatum Vahl against promastigote and amastigote forms of Leishmania amazonensis. The medicinal plant P. ovatum is used popularly as an anesthetic and anti-inflammatory. This study included the extraction process and bioassay-guided fractionation by the adsorption chromatography and Sephadex LH-20 method. A progressive increase in the antileishmanial effect was observed in the course of fractionation. The 50% inhibitory concentration (IC50) for dichloromethane-ethyl acetate (1:1 v/v) fraction was 2.1 μg/ml and 24 μg/ml; mixture of piperovatine: piperlongumune (2:3) 0.9 μg/ml and 24 μg/ml; piperovatine (1) 9.5 μg/ml and 10 μg/ml; and piperlonguminine (2) 2.5 μg/ml and 9.0 μg/ml, for promastigote and amastigote forms, respectively. Cytotoxicity analysis indicated that these toxic concentrations were much higher for J774G8 macrophages and Vero cells than for the protozoans. The mixture of piperovatine: piperlongumune (2:3) showed important antiprotozoal activity against the amastigote and promastigote forms of L. amazonensis, and it produced morphological changes in promastigotes and amastigotes at 0.9 μg/ml and 24 μg/ml (50% growth inhibition concentration), respectively, including intense cytoplasmic vacuolization, mitochondrial swelling, and mitochondrial damage, as revealed by transmission electron microscopy.},
doi = {},
url = {https://ejournals.eu/en/journal/acta-protozoologica/article/in-vitro-antileishmanial-activity-of-hydroalcoholic-extract-fractions-and-compounds-isolated-from-leaves-of-piper-ovatum-vahl-against-leishmania-amazonensis}
}