Abu K. Y. (1996) The phagosome containing Legionella pneumophila within the protozoan Hartmannella vermiformis is surrounded by the rough endoplasmic reticulum. App. Envi. Microbiol. 62: 2022–2028 Brown T. J., Cursons R. T. M., Keys E. A., Marks M., Miles M. (1993) The occurrence and distribution of ecology of pathogenic free-living amebae in thermal areas of the north Island of New Zealand. New Zealand J. Maine Freshwater Res. 17: 59–69 Edagawa A., Kimura A., Kawabuchi-Kurata T., Kusuhara Y., Karanis P. (2009) Isolation and genotyping of potentially pathogenic Acanthamoeba and Naegleria species from tap-water sources in Osaka, Japan. Parasitol. Res. 105: 1109–1117 Ghanchi N. K., Jamil B., Khan E., Ansar Z., Samreen A., Zafar A., Hasan Z. (2017) Case Series of Naegleria fowleri; Primary Ameobic Meningoencephalitis from Karachi, Pakistan. Am. J. Trop. Med. and Hyg. 97: 1600–1602 Greub G., Raoult D. (2004) Microorganisms resistant to free-living amoebae. Clin. Microbiol. Rev. 17: 413–433 John D. T., Howard M. J. (1995) Seasonal distribution of pathogenic free-living amebae in Oklahoma waters. Parasitol. Res. 81: 193–201 Kyle D. E., Noblet G. P. (1985) Vertical distribution of potentially pathogenic free-living amoebae in freshwater lakes. J. Protozool. 32: 99–105 Kyle D. E., Noblet G. P. (1986) Seasonal distribution of thermotolerant free-living amoebae. I. Willard’s Pond. J. Protozool. 33: 422–434 Kyle D. E., Noblet G. P. (1987) Seasonal distribution of thermotolerant free-living amoebae. II. Lake Issaqueena. J. Protozool. 34: 10–15 Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., Lopez R., Thompson J. D., Gibson T. J., Higgins D. G. (2007) ClustalW and ClustalX version 2. Bioinformatics 23: 2947–2948 Maclean R. C., Richardson D. G., LePardo R., Marciano-Cabral F. (2004) The identification of Naegleria fowleri from water and soil samples by nested PCR. Parasitol. Res. 93: 211–217 Marciano-Cabral F., MacLean R., Mensah A., LaPat-Polasko L. (2003) Identification of Naegleria fowleri in domestic water sources by nested PCR. App. Env. Microbiol. 69: 5864–5869 Marciano-Cabral F., Cabral G. A. (2007) The immune response to Naegleria fowleri amebae and pathogenesis of infection. FEMS Immunol. Med. Microbiol. 51: 243–259 Martinez A. J., Visvesvara G . S. (1997) Free-living, amphizoic and opportunistic amebas. Brain Pathol. 7: 583–598 Marshall M. M., Naumovitz D., Ortega Y., Sterling C. R. (1997) Waterborne protozoan pathogens. Clin. Microbiol. Rev. 10: 67–85 Martinez A. J. (2017) Free-living amoebas: natural history, prevention, diagnosis, pathology, and treatment of disease. CRC Press, Boca Raton. 166 (ISBN 9781315893044) Matin A. (2017) Primary amebic meningoencephalitis; a new emerging public health threat by Naegleria fowleri in Pakistan. J. Pharmacol. Res. Drug Design. 1: 1–3 Matin A., Jeong S-Y. (2011) Interaction of Escherichia coli K1 and E. coli K5 with Acanthamoeba castellanii trophozoites and cysts. K J Parasitol. 49: 349–356 Newsome A. L., Scott T. M., Benson R. F., Fields B. S. (1998) Isolation of ameba naturally harboring a distinctive Legionella species. App. Env. Microbiol. 64: 1688–1693 Saleem T., Rabbani M., Jamil B. (2009) Primary amoebic meningoencephalitis: two new cases from Pakistan. Trop. Doc. 39: 242–243 Schuster F. L., Visvesvara G. S. (2004a) Amebae and ciliated protozoa as causal agents of waterborne zoonotic disease. Vet. Parasitol. 126: 91–120 Schuster F. L., Visvesvara G. S. (2004b) Opportunistic amoebae: Challenges in prophylaxis and treatment. Drug Resist. Update. 7: 41–51 Singh B. N. (1975) Pathogenic and non-pathogenic amoebae. Mac-Millan Press Ltd., London. 235 (ISBN: 333-18388-6) Shariq A., Afridi F. I., Farooqi B. J., Ahmed S., Hussain A. (2014) Fatal primary meningoencephalitis caused by Naegleria fowleri. J. Coll. Phys. Surg. Pak. 24: 523–525 Sheehan K. B., Ferris M. J., Henson J. M. (2003) Detection of Naegleria sp. in a thermal, acidic stream in Yellow stone National Park. J. Eukaryot. Microbiol. 50: 263–265 Shakoor S., Beg M. A., Mahmood S. F., Bandea R., Sriram R., Noman F., Ali F., Visvesvara G. S., Zafar A. (2011) Primary Amebic Meningoencephalitis Caused by Naegleria fowleri, Karachi, Pakistan. Emer. Infec. Dis. 17: 258–261 Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evo. 28: 2731–2739 Tanveer T., Hameed A., Dukhyil A. A., Alaidarous M., Matin A. (2017) Isolation of thermotolerant pathogenic Naegleria australiensis from diverse water resources including household drinking water in Pakistan. Acta Protozool. 56: 317–322 Tanveer T., Hameed A., Gul A., Matin A. (2015) Quick survey for detection, identification and characterization of Acanthamoeba genotypes from some selected soil and water samples in Pakistan. Ann. Agri. Env. Med. 22: 232–235 Tanveer T., Hameed A., Muazzam A. G., Jung S-Y., Gul A., Matin A. (2013) Isolation and molecular characterization of potentially pathogenic Acanthamoeba genotypes from diverse water resources including household drinking water from Khyber Pakhtunkhwa, Pakistan. Parasitol. Res. 112: 2925–2932 Tyndall R. L., Ironside K. S., Metler P. L., Tan E. L., Hazen T. C., Fliermans C. B. (1989) Effect of thermal additions on the density and distribution of thermophilic amebae and pathogenic Naegleria fowleri in newly created cooling lake. Appl. Env. Microbiol. 55: 722–732