0065-1583 Jagiellonian University in Kraków Morpho-molecular Characterization of the Litostomatean Predatory Ciliate Phialina pupula (Müller, 1773) Foissner, 1983 (Haptoria, Lacrymariidae) Rajter Ľubomír author Bourland William author Vďačný Peter author Phycology, Faculty of Biology, University of Duisburg-Essen, Essen, Germany Eukaryotic Microbiology, Faculty of Biology, University of Duisburg-Essen, Essen, Germany Boise State University, Department of Biological Sciences, Boise, Idaho, U.S.A. Comenius University, Department of Zoology, Bratislava, Slovakia Correspondence to: Ľubomír Rajter Correspondence to: William Bourland Correspondence to: Peter Vďačný 25 07 2019 Volume 58, Issue 2 2019 53 68 Copyright © 2019 2019 <div>This work was supported by the Slovak Research and Development Agency under the contract No. APVV-15-0147 and by the Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic and Slovak Academy of Sciences under the Grant VEGA 1/0041/17.</div> <p>The morphology and phylogenetic position of a haptorian ciliate, <em>Phialina pupula</em> (Müller, 1773) Foissner, 1983, isolated from microaerobic sandy sediments of the floodplain area of the Boise River, Idaho, U.S.A., were studied using live observation, protargol impregnation, scanning electron microscopy, and the 18S rRNA gene as  well as the ITS region. The Boise population of <em>P. pupula</em> is characterized by a size of about 60–130 × 20–50 μm, an elliptical macronucleus with a single micronucleus, highly refractive dumbbell-shaped inclusions scattered throughout the cytoplasm and concentrated in the anterior body half, a single subterminal/terminal  contractile vacuole, about 10 μm long rod-shaped extrusomes, and an average of 15 ciliary rows. In phylogenetic analyses, the newly obtained sequences from <em>P. pupula </em>and <em>Lacrymaria olor </em>clustered within the family Lacrymariidae with full to moderate statistical support. Neither the genus <em>Phialina </em>nor the genus Lacrymaria was depicted monophyletic both in the single gene and multigene phylogenetic inferences. Specifically, the genus <em>Phialina </em>was shown as a paraphyletic assemblage containing members of the polyphyletic genus <em>Lacrymaria</em>. 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