Morphological and Morphogenetic Redescriptions and SSU rRNA Gene-based Phylogeny of the Poorly-known Species Euplotes amieti Dragesco, 1970 (Ciliophora, Euplotida)
cytuj
pobierz pliki
RIS BIB ENDNOTEChoose format
RIS BIB ENDNOTEMorphological and Morphogenetic Redescriptions and SSU rRNA Gene-based Phylogeny of the Poorly-known Species Euplotes amieti Dragesco, 1970 (Ciliophora, Euplotida)
Publication date: 13.07.2015
Acta Protozoologica, 2015, Volume 54, Issue 3, pp. 171 - 182
https://doi.org/10.4467/16890027AP.15.014.3211Authors
Morphological and Morphogenetic Redescriptions and SSU rRNA Gene-based Phylogeny of the Poorly-known Species Euplotes amieti Dragesco, 1970 (Ciliophora, Euplotida)
This paper investigates the morphology and morphogenesis during binary fission of a Chinese population of Euplotes amieti Dragesco, 1970, a fresh water form which has previously not been well defined. This organism is morphologically very similar to the well-known Euplotes eurystomus but differs from the latter both in the number of dorsal kineties and the molecular data. According to the information obtained, it is characterized by a combination of features including nine frontoventral cirri, ca. 60 membranelles, 12–15 dorsal kineties, a macronucleus in the shape of the number 3, and a ‘double-eurystomus’ type of silverline system. Its morphogenesis proceeds broadly in the same pattern as in its congeners. In this study, the SSU rRNA gene was sequenced for the first time, and phylogenetic analyses indicated that it is closely related to the eurystomus-aediculatus-woodruffi- complex. Considering the extreme similarities in morphology between E. amieti and E.eurystomus, we believe that the four sequences (four isolates) under the name of Euplotes eurystomus (No. FR873716; FR873717; EF193250; AJ310491 deposited in GenBank) are very likely from misidentified material; that is, they represent different populations of Euplotes amieti.
Achilles-Day U. E. M., Pröschold T., Day J. G. (2008) Phylogenetic position of the freshwater ciliate Euplotes daidaleos within the family of Euplotidae, obtained from small subunit rDNA gene sequence. Denisia 23: 411–416
Augustin H., Foissner W. (1989) Morphologie einiger Ciliaten (Protozoa: Ciliophora) aus dem Belebtschlamm. Lauterbornia 1: 38–59
Borror A. C., Hill B. F. (1995) The order Euplotida (Ciliophora): taxonomy, with division of Euplotes into several genera. J. Eukaryot. Microbiol. 42: 457–466
Carter H. P. (1972) Infraciliature of eleven species of the genus Euplotes. Trans. Am. Microsc. Soc. 91: 466–492
Chen X., Zhao Y., Al-Farraj S. A., Al-Quraishy S. A., El-Serehy H. A., Shao C., Al-Rasheid K. A. S. (2013) Taxonomic descriptions of two marine ciliates, Euplotes dammamensis n. sp. and Euplotes balteatus (Dujardin, 1841) Kahl, 1932 (Ciliophora, Spirotrichea, Euplotida), collected from the Arabian Gulf, Saudi Arabia. Acta Protozool. 52: 73–89
Curds C. R. (1975) A guide to the species of the genus Euplotes (Hypotrichida, Ciliatea). Bull. Br. Mus. Nat. Hist. (Zool.). 28: 3–61
Curds C. R., Wu I. C. H. (1983) A review of the Euplotidae (Hypotrichida, Ciliophora). Bull. Br. Mus. Nat. Hist. (Zool.). 44: 191–247
Dai R., Xu K., He Y. (2013) Morphological, physiological, and molecular evidences suggest that Euplotes parawoodruffi is a junior synonym of Euplotes woodruffi (Ciliophora, Euplotida). J. Eukaryot. Microbiol. 60: 70–78
Dragesco J. (1970) Ciliés libres du Cameroun. Ann. Fac. Sci. Jaoundé (Hors série), 1–141
Dragesco J. (1972) Ciliés libres de l’Ouganda. Ann. Fac. Sci. Univ. Féd. Cameroun 9: 87–126
Dragesco J. (2003) Infraciliature et morphometrie de vingt espèces de ciliés hypotriches recoltés au Rwanda et Burundi, comprenant kahliella quadrinucleata n. sp., pleurotricha multinucleata n. sp. et laurentiella bergeri n. sp. Trav. Mus. Nat. d’Hist. Na
Dragesco J., Dragesco-Kernéis A. (1986) Ciliés libres de l’Afrique intertropicale. Introduction à la connaissance et à l’étude des ciliés. Faune Tropicale 26: 495–497
Edgar R. C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32: 1792–1797
Fan Y., Chen X., Hu X., Shao C., Al-Rasheid K. A. S., Al-Farraj S. A., Lin X. (2014) Morphology and morphogenesis of Apoholosticha sinica n. g., n. sp. (Ciliophora, Hypotrichia), with consideration of its systematic position among urostylids. Eur. J. Protistol. 50: 78–88
Foissner W. (1982) Ecology and taxonomy of the Hypotrichida (Protozoa: Ciliophora) of some Austrian soils. Arch. Protistenkd. 126: 19–143
Foissner W. (2014) An update of ‘basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa’. Int. J. Syst. Evol. Microbiol. 64: 271–292
Foissner W., Blatterer H., Berger H., Kohmann F. (1991) Taxonomische und ökologische Revision der Ciliaten des Saprobiensystems – Band I: Cyrtophorida, Oligotrichida, Hypotrichia, Colpodea. Informationsberichte des Bayer, Landesamtes für Wasserwirtschaft, München, 1/91: 1–478
Gates M. A., Curds C. R. (1979) The dargyrome of the genus Euplotes. Bull. Br. Mus. Nat. Hist. (Zool). 35: 127–200
Hall T. A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41: 95–98
Huang J., Dunthorn M., Song W. (2012) Expanding character sampling for the molecular phylogeny of euplotid ciliates (Protozoa, Ciliophora) using three markers, with a focus on the family Uronychiidae. Mol. Phylogent. Evol. 63: 598–605
Huang J., Chen Z., Song W., Berger H. (2014) Three-gene based phylogeny of the Urostyloidea (Protista, Ciliophora, Hypotricha), with notes on classification of some core taxa. Mol. Phylogent. Evol. 70: 337–347
Jiang J., Huang J., Li J., Al-Rasheid K. A. S., Al-Farraj S. A., Lin X., Hu X. (2013) Morphology of two marine euplotids (Ciliophora: Euplotida), Aspidisca fusca Kahl, 1928 and A. hexeris Quennerstedt, 1869, with notes on their small subunit rRNA gene sequences. Eur. J. Protistol. 49: 634–643
Jiang J., Zhang Q., Warren A., Al-Rasheid K. A. S., Song W. (2010) Morphology and SSU rRNA gene-based phylogeny of two marine Euplotes species, E. orientalis spec. nov. and E. raikovi Agamaliev, 1966 (Ciliophora, Euplotida). Eur. J. Protistol. 46:
Lynn D. H. (2008) The ciliated protozoa: characterization, classification and guide to the literature. 3rd ed. Springer Press, Dordrecht
Ma H., Gong J., Wang Y., Hu X., Ma H., Song W. (2000) Morphological studies on Euplotes eurystomus (Ciliophora, Hypotrichida) compared with its related species from freshwater biotopes. J. Zibo Univ. (Nat. Sci. and Eng. Ed.) 2: 75–77 (in Chinese with English abstract)
Medlin L., Elwood H. J., Stickel S., Sogin M. L. (1988) The characterization of enzymatically amplified eukaryotes 16S-like ribosomal RNA coding regions. Gene 71: 491–500
Nylander J. A. A. (2004) MrModeltest version 2. Program distributed by the author. Department of Systematic Zoology, Evolutionary Biology Centre, Uppsala University, Uppsala
Page R. D. M. (1996) TREEVIEW: an application to view phylogenetic trees on personal computers. Comput. Appl. Biosci. 12: 357–358
Pan Y., Li L., Shao C., Hu X., Ma H., Al-Rasheid K. A. S., Warren A. (2012) Morphology and ontogenesis of a marine ciliate, Euplotes balteatus (Dujardin, 1841) Kahl, 1932 (Ciliophora, Euplotida) and definition of Euplotes wilberti nov. spec. Acta Protozool. 51: 29–38
Pang Y., Wei H. (1999) Studies on the Morphology and Morphogenesis in Euplotes aediculatus. J. East China Normal Univ. (Natural Science) 1: 103–109 (in Chinese with English abstract)
Petroni G., Dini F., Verni F., Rosati G. (2002) A molecular approach to the tangled intrageneric relationships underlying phylogeny in Euplotes (Ciliophora, Spirotrichea). Mol. Phylogenet. Evol. 22: 118–130
Pfeiffer W., Stamatakis A. (2010) Hybrid parallelization of the MrBayes and RAxML phylogenetics codes.
Pierson D. M., Kimball R. F., Carrier R. F. (1968) Clarification of the taxonomic identification of Euplotes eurystomus Kahl and E. aediculatus Pierson. Trans. Am. Microsc. Soc. 72: 125–151
Posada D., Crandall K. A. (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14: 817–818
Pratas F., Trancoso P., Stamatakis A., Sousa L. (2009) Fine-grain parallelism using Multi-core, Cell/BE, and GPU systems: accelerating the phylogenetic likelihood function. Proceedings of ICPP Vienna, Austria.
Ronquist F., Huelsenbeck J. P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574
Schwarz M., Zuendorf A., Stoeck T. (2007) Morphology, ultrastructure, molecular phylogeny, and autecology of Euplotes elegans Kahl, 1932 (Hypotrichida; Euplotidae) isolated from the Anoxic Mariager Fjord. Denmark. J. Eukaryot. Microbiol. 54: 125–136
Shao C., Ding Y., Al-Rasheid K. A. S., Al-Farraj S. A., Warren A., Song W. (2013a) Establishment of a new hypotrichous genus, Heterotachysoma n. gen. and notes on the morphogenesis of Hemigastrostyla enigmatica (Ciliophora, Hypotrichia). Eur. J. Protistol. 49: 93–105
Shao C., Pan X., Jiang J., Ma H., Al-Rasheid K. A. S., Warren A., Lin X. (2013b) A redescription of the oxytrichid Tetmemena pustulata (Müller, 1786) Eigner, 1999 and notes on morphogenesis in the marine urostylid Metaurostylopsis salina Lei et al., 2005 (Ciliophora, Hypotrichia). Eur. J. Protistol. 49: 272–282
Song W., Bradbury P. (1997) Comparative studies on a new brackish water Euplotes, E. parawoodruffi n. sp., and a redescription of Euplotes woodruffi GAW, 1939 (Ciliophora; Hypotrichida). Arch. Protistenkd. 148: 399–412
Song W., Warren A., Hu X. (2009) Free-living ciliates in the Bohai and Yellow Seas, China. Science Press, Beijing
Stamatakis A. (2006) RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690
Stamatakis A., Hoover P., Rougemont J. (2008) A fast bootstrapping algorithm for the RAxML web-servers. Syst. Biol. 57: 758–771
Tamura K., Dudley J., Nei M., Kumar S. (2007) MEGA 4: molecular evolutionary genetics analysis (MEGA) software Ver. 4.0. Mol. Biol. Evol. 24: 1596–1599
Tuffrau M. (1960) Révision du genre Euplotes, fondée sur la comparaison des structures superficielles. Hydrobiologia 15: 1–77
Wilbert N. (1975) Eine verbesserte Technik der Protargolimprägnation für Ciliaten. Mikrokosmos 64: 171–179
Yi Z., Song W., Clamp J., Chen Z., Gao S., Zhang Q. (2009) Reconsideration of systematic relationships within the order Euplotida (Protista, Ciliophora) using new sequences of the gene coding for small-subunit rRNA and testing the use of combined data sets to construct phylogenies of the Diophrys-like species. Mol. Phylogent. Evol. 50: 599–607
Yi Z., Katz L. A., Song W. (2012) Assessing whether alpha-tubulin sequences are suitable for phylogenetic reconstruction of Ciliophora with insights into its evolution in euplotids. PLoS ONE 7: e40635. doi:10.1371/journal.pone.0040635
Information: Acta Protozoologica, 2015, Volume 54, Issue 3, pp. 171 - 182
Article type: Original article
Laboratory of Protozoology, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, China
Laboratory of Protozoology, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, China
Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Laboratory of Protozoology, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, China; Department of Zoology, Natural History Museum, Cromwell Road, London, UK
Zoology Department, King Saud University, Riyadh, Saudi Arabia
Laboratory of Protozoology, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, China
Laboratory of Protozoology, Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, China; Department of Zoology, Natural History Museum, Cromwell Road, London, UK
Published at: 13.07.2015
Article status: Open
Licence: None
Percentage share of authors:
Article corrections:
-Publication languages:
English